RETICULOENDOTHELIAL FUNCTION IN PATIENTS WITH AIDS

艾滋病患者的网状内皮功能

• 批准号: 4688498
• 负责人: T C QUINN
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4688498
• 关键词: AIDS; Actinomycetales infection; T lymphocyte; cell mediated cytotoxicity; homosexuals; human subject; immunologic assay /test; immunopathology diagnosis; longitudinal human study; lymphadenectomy; male; monocyte; phagocytic dysfunction; phagocytosis; reticuloendothelial system

The acquired immune deficiency syndrome (AIRS) is characterized by multiple opportunistic infections which are normally killed by phagocytic cells of the reticuloendothelial system (RES). We have prospectively analysed in vivo RES function by measureing Fc receptor clearance and C3b-receptor clearance in AIDS patients and patients at risk for AIDS. In vivo RES clearance defects. A total of 63 patients have been studies, including 22 AIDS patients, 9 patients with AIDS-related complex, 13 healthy homosexual men, 15 healthy heterosexuals and 4 heterosexuals with mycobacterial disease but without AIDS. Eleven of fifteen AIDS patients and 2 of 9 with AIDS-related complex had prolonged Fc specific clearance rates compared to controls. In contrast, patients with mycobacterial infection but without AIDS had significantly more rapid clearance rates reflective of activated macrophage function. Similarly, C3b clearance rates were markedly abnormal in 7 AIDS patients compared to controls. Patients with AIDS had a relatively large initial clearance of C3b labeled cells, but unlike controls, they had a relatively large release of cells back in circulation. These defects of both Fc and C3b clearance rates suggest a marked phagocytic defect of the RES. In vitro phagocytic defects. In order to measure the degree of phagocytic defect, we investigated antibody directed cell mediated cytoxicity (ADCC) of peripheral blood mononuclear cells of patients with AIDS against chicken red blood cells (CRBC). Compared to healthy controls, a marked decrease in ADCC-CRBC activity was observed in mononuclear cells from AIDS patients. No suppression in ADCC activity was seen when mononuclear cells from healthy subjects were assayed using media containing 10% sera from AIDS patients. The significance of this project is in the identification of a marked phagocytic defect both in vivo and in vitro in patients with AIDS. Further studies will examine the severity and characterization of the phagocytic defect.

获得的免疫缺陷综合征（AIRS）的特征是多个 机会性感染通常被吞噬细胞杀死 网状内皮系统（RES）。 我们已经前瞻性地分析了 通过测量FC受体清除率和C3B受体的体内RES功能 艾滋病患者和有艾滋病风险的患者清除。 体内弥补清除缺陷。 总共有63名患者正在研究， 包括22名艾滋病患者，9名与艾滋病相关复合物患者，13例 健康的同性恋男性，15位健康异性恋和4个异性恋者 分枝杆菌疾病，但没有艾滋病。 十五名艾滋病患者中有11位 与艾滋病相关复合物的9中的2个中有延长了FC特异性间隙 与对照组相比。 相反，分枝杆菌患者 感染但没有艾滋病的清除率明显更快 反映活化的巨噬细胞功能。 同样，C3B清除率 与对照组相比，7名艾滋病患者的率明显异常。 艾滋病患者的标记为C3B的初始清除率相对较大 细胞，但与对照不同，它们的细胞释放相对较大 回到循环。 FC和C3B清除率的这些缺陷 建议RES的明显吞噬缺陷。 体外吞噬缺陷。 为了测量吞噬的程度 缺陷，我们研究了抗体定向细胞介导的细胞毒性（ADCC） 用辅助鸡的患者的外周血单核细胞的 红细胞（CRBC）。 与健康对照相比，明显下降 在艾滋病患者的单核细胞中观察到ADCC-CRBC活性。 当来自的单核细胞来自 使用含有10％艾滋病血清的培养基对健康受试者进行测定 患者。 该项目的意义在于标记 艾滋病患者的体内和体外吞噬缺陷均可吞噬。 更远 研究将检查吞噬细胞的严重程度和表征 缺点。