CYTOPATHOLOGY OF ASBESTOS AND OTHER FIBERS ON HUMAN LUNG CELLS IN CULTURE

石棉和其他纤维对培养的人肺细胞的细胞病理学

• 批准号: 4692385
• 负责人: E W GABRIELSON
• 金额: --
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4692385
• 关键词: X ray crystallography; asbestos; bronchus; carcinogen testing; cell population study; cell type; cellular oncology; chemical carcinogen; chemical carcinogenesis; chromosome disorders; cytodiagnosis; cytotoxicity; electron microscopy; electron probe spectrometry; environmental toxicology; fibroblasts; glass; histopathology; human tissue; neoplasm /cancer diagnosis; neoplasm /cancer genetics; particle; phagocytosis; phase contrast microscopy; physical chemical interaction; respiratory epithelium; tissue /cell culture

Our laboratory is studying the cytopathology of asbestos fibers on human bronchial epithelial cells, human mesothelial cells and human fibroblasts in culture. Electron microscopy studies have demonstrated phagocytosis of the fibers and phase-microscopy of metaphase spreads reveal frequent attachment of the fibers to the chromosomes. Investigations regarding the mechanism of asbestos carcinogenesis suggest that oxygen radicals are probably not important intermediates. Further studies have examined the cytotoxicity of synthetic fibers, i.e., glass and carbon-graphite, on human mesothelial cell cultures.

本实验室正在研究石棉纤维对人体的细胞病理学作用 支气管上皮细胞、人间皮细胞和人成纤维细胞 在文化中。 电子显微镜研究表明， 中期铺展的纤维和相显微镜显示， 纤维附着在染色体上。 调查 石棉致癌机制表明，氧自由基是 可能不是重要的中间体。 进一步的研究检查了 合成纤维的细胞毒性，即，玻璃和碳-石墨，对人体 间皮细胞培养物。