TRANSFORMING GENES OF AVIAN RNA TUMOR VIRUSES

我们也在研究病毒致癌基因 作为它们的胶质同源物，诱导生化事件的激活， 引导细胞向恶性转化和瘤形成。 利用 逆转录病毒作为了解病毒结构和分子本质的工具 由这些病毒转导的细胞癌基因使得 这些细节在正常和恶性系统中的分辨率。 此外，本发明还 我们利用这些onc基因作为探针， myc和mht原癌基因在多种细胞中的组织，如 从人类一直进化到鱼类和果蝇 此外 研究三种逆转录病毒（MC 29，MH 2 和OK10），都是myc癌基因家族的一部分，我们已经接近了 组织几个双oncoene系统，试图解决 MH2中基因mht和myc以及E26中基因ets和myb的致癌相互作用。 利用这些探针使我们能够扩大我们的研究，包括 ets和myc在几种肿瘤组织中的组织和表达 会发生染色体重排 此外，我们还有 构建并利用表达载体来产生大量的 特定癌基因产物，然后对其进行纯化和表征 在化学和免疫学上， 过程中的试剂。 此外，我们对分子结构的了解 使我们能够制造独特的合成寡肽 并产生同源抗血清来探测这些相对稀缺的蛋白质， 正常的细胞系统，这导致了它们的细胞内定位 和细胞联系。 这些关于正常和激活的癌基因的研究 将有重要的诊断，以及功能， 了解它们在恶性肿瘤过程中的作用。

We are pursuing the study of the process by which viral oncogenes, as well as their collular homolgs, induce the activation of biochemical events that direct the cell towards malignant transformation and neoplasia. Utilizing retroviruses as tools to understand the structural and molecular nature of the cellular oncogenes transduced by these viruses has enabled the resolution of these details in both normal and malignant systems. Further, we have utilized these onc genes as probes revealing the genomic organization of myc and mht proto-oncogenes in a variety of cells, such as human, all the way down evolutionarily to fish and Drosophila. In addition to studying the genomic organization of the three retroviruses (MC29, MH2 and OK10), all part of the myc oncogene family, we have approached the organization of several dual oncoagene systems in an attempt to resolve the oncogenic interaction of genes mht and myc in MH2, and ets and myb in E26. Utilizing these probes has enabled us to expand our study to include the organization and expression of ets and myc in several human neoplasias which undergo chromosomal rearrangements. Additionally, we have constructed and utilized exapression vectors to produce large amounts of specific oncogene products and then to purify and characterize them chemically and immunologically, producing valuable immunodiagnostic reagents in the process. Further, our knowledge of the molecular structure of specific oncogenes has enabled us to make unique synthetic oligopeotides and produce cognate antisera to probe these relatively scarce proteins in normal cellular systems, which has led to their intracellular localization and cellular association. These studies on normal and activate oncogenes will have inportant diagonostic, as well as functional, implications towards understanding their role in the process of malignancy.