Post-transcriptional control of gene expression following toxic injury

毒性损伤后基因表达的转录后控制

• 批准号: MC_UU_00025/7
• 负责人: Anne Willis
• 金额: $ 324.54万
• 依托单位: University of Cambridge
• 依托单位国家: 英国
• 项目类别: Intramural
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=MC_UU_00025%2F7
• 关键词: Post; transcriptional; control; gene; expression

Protein synthesis is the process by which the information in the genetic material in the cell, DNA is converted via an intermediary substrate called mRNA, into proteins. For proteins to be made the mRNA must interact with a large complex called the ribosome which consists of RNAs and proteins. Ribosomes can therefore be thought of as “molecular factories” that make proteins. They do this by decoding the genetic information that is held in the mRNA and bringing all the building blocks together to synthesise proteins. The rate at which proteins are made is very highly regulated and cells respond to alterations in the external environment, such as exposure to toxic chemicals and chemotherapeutic agents by modifying both the rate at which they make proteins and importantly the types of proteins that they make. We are studying these processes to gain a greater understanding of the cells/organisms response to toxic insult.

蛋白质合成是细胞遗传物质中的信息，DNA通过称为mRNA的中间底物转化为蛋白质的过程。为了制造蛋白质，mRNA必须与一个称为核糖体的大复合体相互作用，核糖体由RNA和蛋白质组成。因此，核糖体可以被认为是制造蛋白质的“分子工厂”。它们通过解码mRNA中的遗传信息并将所有构建模块组合在一起来合成蛋白质。蛋白质制造的速率受到高度调节，细胞对外部环境的改变做出反应，例如通过改变它们制造蛋白质的速率以及重要的是它们制造的蛋白质类型来暴露于有毒化学品和化疗剂。我们正在研究这些过程，以更好地了解细胞/生物体对有毒物质的反应。

项目成果

Development of a colorimetric assay for the detection of SARS-CoV-2 3CLpro activity.

• DOI: 10.1042/bcj20220105
• 发表时间: 2022-04-29
• 期刊: The Biochemical journal

Crosstalk with lung fibroblasts shapes the growth and therapeutic response of mesothelioma cells.

• DOI: 10.1038/s41419-023-06240-x
• 发表时间: 2023-11-08
• 期刊: CELL DEATH & DISEASE
• 影响因子: 9
• 作者: Chrisochoidou, Yakinthi;Roy, Rajat;Farahmand, Pooyeh;Gonzalez, Guadalupe;Doig, Jennifer;Krasny, Lukas;Rimmer, Ella F.;Willis, Anne E.;MacFarlane, Marion;Huang, Paul H.;Carragher, Neil O.;Munro, Alison F.;Murphy, Daniel J.;Veselkov, Kirill;Seckl, Michael J.;Moffatt, Miriam F.;Cookson, William O. C.;Pardo, Olivier E.
• 通讯作者: Pardo, Olivier E.

The pathogenesis of mesothelioma is driven by a dysregulated translatome.

• DOI: 10.1038/s41467-021-25173-7
• 发表时间: 2021-08-13
• 期刊: Nature communications
• 影响因子: 16.6
• 作者: Grosso S;Marini A;Gyuraszova K;Voorde JV;Sfakianos A;Garland GD;Tenor AR;Mordue R;Chernova T;Morone N;Sereno M;Smith CP;Officer L;Farahmand P;Rooney C;Sumpton D;Das M;Teodósio A;Ficken C;Martin MG;Spriggs RV;Sun XM;Bushell M;Sansom OJ;Murphy D;MacFarlane M;Le Quesne JPC;Willis AE
• 通讯作者: Willis AE

Extracellular Vesicles Isolated from Malignant Mesothelioma Cancer-Associated Fibroblasts Induce Pro-Oncogenic Changes in Healthy Mesothelial Cells.

• DOI: 10.3390/ijms232012469
• 发表时间: 2022-10-18
• 期刊: International journal of molecular sciences
• 影响因子: 5.6

Trans-acting translational regulatory RNA binding proteins.

• DOI: 10.1002/wrna.1465
• 发表时间: 2018-05
• 期刊: Wiley interdisciplinary reviews. RNA
• 作者: Harvey RF;Smith TS;Mulroney T;Queiroz RML;Pizzinga M;Dezi V;Villenueva E;Ramakrishna M;Lilley KS;Willis AE
• 通讯作者: Willis AE