TRANSGENIC MOUSE MODEL SYSTEM FOR THE ETS-1 AND ETS-2 PROTO-ONCOGENE FUNCTION

ETS-1 和 ETS-2 原癌基因功能的转基因小鼠模型系统

• 批准号: 5201517
• 负责人: J LAUTENBERGER
• 金额: --
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5201517
• 关键词: bone marrow; cytokine receptors; gene expression; genetic promoter element; genetic regulation; genetically modified animals; hematopoiesis; interleukin 3; laboratory mouse; megakaryocytes; metallothionein; protooncogene; spleen; tissue /cell culture; transcription factor

The effect of transgene expression on the regulation of hematopoiesis was examined in transgenic mice in which the Ets2 gene was driven by the mouse metallothionein promoter. It has been found that transgene expression results in an increase in the number of cells from bone marrow and spleen capable of forming colonies in semisolid media containing IL-3. The transgenic mice were found to have a higher percentage of megakaryocytes in their bone marrow than control animals. Bone marrow cultures derived from transgenic animals express higher levels of the c-mpl gene as compared to normal controls. c-mpl codes for the receptor for the platelet-inducing cytokine, thrombopoietin.

转基因表达对造血调控的影响 在转基因小鼠中进行检测，其中Ets2基因由 小鼠金属硫蛋白启动子。现已发现，转基因 表达导致来自骨髓的细胞数量增加 和脾能够在半固体介质中形成克隆，包括 IL-3。转基因小鼠被发现有更高比例的 大鼠骨髓中的巨核细胞数量高于对照组动物。骨髓 来自转基因动物的培养表达了更高水平的 C-mpl基因与正常对照比较。受体的C-MPL代码 血小板诱导细胞因子--促血小板生成素。