BIOCHEMICAL AND FUNCTIONAL PROPERTIES OF THE ETS PROTO-ONCOGENES

人类免疫缺陷病毒（HIV-1和HIV-2），沿着其 猴免疫缺陷病毒（SIV）属于 慢病毒家族，并且是获得性免疫的病原体。 缺乏综合征（艾滋病）。 HIV-1和HIV-2 mRNA的调控 表达由位于长链中的顺式调节序列决定。 前病毒的末端重复（LTR）区。 HIV-1 LTR含有一个 核心增强子位于-79至-109 bp处，其具有重叠NF κ B 和ETS结合位点（EBS）（GGGACTTTCC）在一个直接重复 配置. 含有双聚体或多聚体的启动子和增强子的检查 EBS揭示了EBS存在两个不同的方向：i）直接， 其中富含嘌呤的DNA链具有线性的“头到尾” 方向，和ii）回文，其中EBS被发现存在于 “头对头”的定向。 两个EBS核心之间的间距， HIV-1增强子是10个核苷酸，然而，两个增强子之间的距离 EBS核心在不同的启动子中存在差异。

The human immunodeficiency viruses (HIV-1 and HIV-2), along with their simian counterpart, simian immunodeficiency virus (SIV), belong to the lentivirus family and are the etiological agents of acquired immune deficiency syndrome (AIDS). The regulation of HIV-1 and HIV-2 mRNA expression is determined by cis-regulatory sequences located in the long terminal repeat (LTR) region of the provirus. The HIV-1 LTR contains a core enhancer located at -79 to -109 bp, which has overlapping NFkappaB and ETS binding sites (EBS) (GGGACTTTCC) in a direct repeat configuration. An examination of promoters and enhancers that contain dual or multimeric EBS reveals that two distinct orientations exist for EBS: i) direct, in which the purine rich DNA strand has a linear, "head to tail" orientation, and ii) palindromic, in which the EBS are found to exist in a "head to head" orientation. The spacing between the two EBS cores in the HIV-1 enhancer is ten nucleotides, however, the distance between two EBS cores varies in different promoters.