BIOCHEMICAL AND FUNCTIONAL PROPERTIES OF THE ETS PROTO-ONCOGENES

ETS 原癌基因的生化和功能特性

• 批准号: 5201515
• 负责人: J LAUTENBERGER
• 金额: --
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5201515
• 关键词: AIDS; DNA binding protein; chemical binding; gene expression; genetic enhancer element; genetic promoter element; genetic regulation; human immunodeficiency virus 1; human immunodeficiency virus 2; human tissue; messenger RNA; molecular site; nucleic acid repetitive sequence; nucleotides; oncoproteins; protein structure function; protooncogene; provirus; simian immunodeficiency virus; transcription factor

The human immunodeficiency viruses (HIV-1 and HIV-2), along with their simian counterpart, simian immunodeficiency virus (SIV), belong to the lentivirus family and are the etiological agents of acquired immune deficiency syndrome (AIDS). The regulation of HIV-1 and HIV-2 mRNA expression is determined by cis-regulatory sequences located in the long terminal repeat (LTR) region of the provirus. The HIV-1 LTR contains a core enhancer located at -79 to -109 bp, which has overlapping NFkappaB and ETS binding sites (EBS) (GGGACTTTCC) in a direct repeat configuration. An examination of promoters and enhancers that contain dual or multimeric EBS reveals that two distinct orientations exist for EBS: i) direct, in which the purine rich DNA strand has a linear, "head to tail" orientation, and ii) palindromic, in which the EBS are found to exist in a "head to head" orientation. The spacing between the two EBS cores in the HIV-1 enhancer is ten nucleotides, however, the distance between two EBS cores varies in different promoters.

人类免疫缺陷病毒（HIV-1 和 HIV-2）及其病毒 猿猴对应物，猿猴免疫缺陷病毒（SIV），属于 慢病毒家族，是获得性免疫的病原体 缺乏综合症（艾滋病）。 HIV-1 和 HIV-2 mRNA 的调控 表达由位于长链的顺式调控序列决定 原病毒的末端重复（LTR）区域。 HIV-1 LTR 包含 核心增强子位于-79至-109 bp，具有重叠的NFkappaB 和 ETS 结合位点 (EBS) (GGGACTTTCC) 直接重复 配置。 对含有双或多聚体的启动子和增强子的检查 EBS 揭示了 EBS 存在两个不同的方向：i) 直接、in 其中富含嘌呤的 DNA 链具有线性、“从头到尾” 方向，ii) 回文，其中 EBS 被发现存在于 “头对头”的方向。 两个EBS核心之间的间距 HIV-1增强子是十个核苷酸，然而，两个之间的距离 EBS 核心在不同的启动子中有所不同。