MOLECULAR MARKERS OF HUMAN LIVER CANCER-NOVEL GENES DIFFERENTIALLY EXPRESSED
MOLECULAR MARKERS OF HUMAN LIVER CANCER-NOVEL GENES DIFFERENTIALLY EXPRESSED
批准号:
5201562
负责人:
J LAUTENBERGER
金额:
$0.0万
依托单位国家:
美国
项目类别:
财政年份:
--
资助国家:
美国
项目状态:
未结题
起止时间:
至
关键词:
antibody complementary DNA computer assisted sequence analysis endoplasmic reticulum gene expression genetic markers histones human genetic material tag human tissue liver neoplasms membrane proteins messenger RNA molecular oncology neoplasm /cancer genetics northern blottings nucleic acid repetitive sequence nucleoproteins polymerase chain reaction protein sequence secretory protein tissue /cell culture transcription factor
中文摘要
我们以前已经表明,粗面内质网(RER)来自
英文摘要
We have previously shown that rough endoplasmic reticulum (RER) from
liver cell lines can give intact and enriched secretory protein mRNA (See
project #Z01CP05739-01 LMO). Such mRNA was used as part of an assay
system to determine which, if any, 3' cDNA fragments cloned by
differential display correspond to secreted proteins.
Out of 20 differentially expressed cDNA fragments, one was found novel
by database sequence analysis and corresponding to a secretory protein
by RER fraction-extracted RNA Northern analysis. We used 5' rapid
amplification of cDNA ends (RACE) polymerase chain reaction (PCR) to
clone the entire 3.7 kb mRNA from placenta mRNA, which was the only
healthy tissue that expressed this novel clone. This gene contains a
leader peptide sequence, a proline repeat, and seven potential
membrane-spanning domains, according to computer analysis. The other 19
differentially expressed clones contain six complete novel genes, a
histone-related gene, a transcriptional factor, and nuclear cytosolic
proteins which are negative for RER localization, according to the
previously discussed assay. We are currently focusing on our putative
cell surface protein clone for protein expression, antibody production
and patient sera/tissue screening to determine its diagnostic/
prognostic/therapeutic significance.
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会议论文
ETS ONCOGENE EXPRESSION IN BACTERIA AND YEAST
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批准号:3916926
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:J LAUTENBERGER
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依托单位:
BIOCHEMICAL AND FUNCTIONAL PROPERTIES OF THE ETS PROTO-ONCOGENES
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批准号:5201515
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:J LAUTENBERGER
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依托单位:
TUMOR SUPPRESSOR ACTIVITIES OF ETS1 GENE PRODUCTS
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批准号:5201556
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:J LAUTENBERGER
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依托单位:
REAL-TIME ASSESSMENT OF MACROMOLECULAR INTERACTIONS BY SURFACE PLASMON RESONANCE
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批准号:5201598
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:J LAUTENBERGER
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依托单位:
MOLECULAR ASPECTS OF COLON EPITHELIUM DIFFERENTIATION AND TUMOR FORMATION
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批准号:3752747
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:J LAUTENBERGER
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依托单位:
TRANSGENIC MOUSE MODEL SYSTEM FOR THE ETS-1 AND ETS-2 PROTO-ONCOGENE FUNCTION
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批准号:5201517
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:J LAUTENBERGER
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依托单位:
海外基金