VIRUS INFECTIONS IN THE EYE

我们对各种病毒学和免疫病理过程的研究 当病毒和寄生虫在眼睛里复制时发生 微环境由五个方面组成：(1)病毒诱导的视网膜 退化过程，(2)病毒在人类中的可能作用 疾病；(3)巨细胞病毒的分子诊断和致病机制 (4)水痘-带状疱疹病毒(VZV) 视网膜感染，以及(5)弓形虫感染 视网膜。 我们已经证实，小鼠冠状病毒可以引起眼部疾病 可作为研究视网膜退行性变的模型系统 疾病。这款车有很多独特的特点。该病毒能够 在有轻度视网膜血管的情况下引起急性感染 发炎。最初的视网膜损伤之后是清除 传染性病毒和进行性视网膜变性。视网膜 这种疾病的退化期有持久的病毒成分， 一种免疫成分，一种遗传成分。关于中国石油天然气集团公司 视网膜变性的免疫学成分表明 病毒RNA和病毒蛋白定位于(细胞)视网膜色素 被激活的上皮(RPE)和睫状体上皮细胞 表达主要组织相容性复合体(MHC)分子和 黏附分子。这些数据表明，自身免疫反应性 可在此站点启动。 这种疾病可以被认为是一种退行性疾病的模型。 人类的色素上皮和光感受器。 人类巨细胞病毒(CMV)是一种疱疹病毒，是引起 先天感染和先天感染儿童的失明 免疫功能受损的个体。CMV潜伏期的研究是很困难的 天哪。因此，CMV复制和潜伏期的细胞培养模型可能 提供对替代治疗方式的基本原理的见解。 我们鉴定了巨细胞病毒在人RPE细胞中复制。然而，在那里 RPE细胞中的病毒复制有几个明显的差异 与人类成纤维细胞相比。这些可能是 病毒在视网膜内的持续和激活。

Our studies of various virologic and immunopathologic processes that occur when viruses and parasites replicate in the ocular microenvironment make up five areas: (1) virus-induced retinal degenerative processes, (2) the possible roles of viruses in human diseases, (3) molecular diagnosis and pathogenesis of cytomegalovirus (CMV) infections in humans, (4) varicella-zoster virus (VZV) infections of the retina, and (5) Toxoplasma gondii infections of the retina. We have established that murine coronavirus can induce ocular disease and may be used as a model system for studying retinal degenerative diseases. This model has many unique features. The virus is capable of inducing an acute infection in the presence of mild retinal vascular inflammation. Initial retinal damage is followed by clearance of infectious virus and progressive retinal degeneration. The retinal degenerative phase of this disease has a persistent virus component, an immune component, and a genetic component. Studies on the immunologic component of retinal degeneration demonstrate that the viral RNA and viral proteins are localized in (cells retinal pigment epithelium (RPE) and ciliary body epithelial cells) that are activated to express major histocompatibility complex (MHC) molecules and adhesion molecules. These data suggest that the autoimmune reactivity may be initiated at this site. This disease may be considered a model for degenerative diseases of the pigment epithelium and photoreceptors in humans. Human cytomegalovirus (CMV) is a herpesvirus that is a major cause of blindness in children born with congenital infections and in immunocompromised individuals. It is difficult to study CMV latency in man. Therefore, cell culture models of CMV replication and latency may provide insight into a rationale for alternative treatment modalities. We identified that CMV replicates in human RPE cells. However, there are a number of distinct differences in virus replication in RPE cells in comparison to human fibroblasts. These may be critical variables in viral persistence and viral activation within the retina.