IDENTIFICATION OF GENOMIC CHANGES MEDIATING MELANOMA DEVELOPMENT AND PROGRESSION

这个项目的目的仍然是确定 染色体重排的分子基础 人类恶性黑色素瘤的进展。黑色素瘤是最快的 任何癌症发病率的增长速度(仅次于女性肺癌) 在西方世界，人们对此知之甚少 这种重要疾病背后的分子遗传变化。这 项目可以分为三个组成部分。1)分子分析 肿瘤的进展。[这集中在克隆进展上，由 组织解剖和比较基因组杂交，以及克隆 恶性黑色素瘤的核型改变]。2)识别 在生长、分化和发育过程中差异表达的基因 进步。[这集中在抑制肿瘤的致瘤性上 6号染色体，cDNA差减/差异显示，以及 逆转录病毒介导的肿瘤抑制逆转]。3)基因组分析 6号染色体的改变。[这涉及基于显微解剖的 黑色素瘤和其他恶性肿瘤的染色体断裂点的克隆]。

The purpose of this project continues to be the determination of the molecular basis of chromosome rearrangements underlying the genesis and progression of human malignant melanoma. Melanoma has the fastest growing rate of incidence of any cancer (next to lung cancer in women) in the Western world, and extraordinarily little is known about the molecular genetic changes underlying this important disease. This project can be divided into three component parts. 1) Molecular analysis of tumor progression. [This focuses on clonal progression identified by tissue dissection and comparative genomic hybridization, and on clonal karyotypic alterations in malignant melanoma]. 2) Identification of genes differentially expressed during growth, differentiation and progression. [This focuses on suppression of tumorigenicity of chromosome 6, cDNA subtraction/differential display, and retroviral-mediated reversion of tumor suppression]. 3) Genomic analysis of chromosome 6 alterations. [This involves microdissection-based cloning of chromosomal breakpoints in melanoma, and other malignancies].