CORE--MOLECULAR RESOURCES

我们建议建立一个分子资源核心实验室(MRCL)， 提供基本试剂、服务和服务的具体目的 协助采用方法学支持《联合国宪章》的六个分构成部分 斯科尔。该MRCL被设计为一个集中式设施，用于 仪器、专门的分子生物学专业知识和试剂 以使它极具成本效益和高效率。MRCL 将帮助设计和合成用于 引子、探针和反义试剂。我们还将提供合成的 寡肽和这些多肽的位点特异性变体用作 免疫原和作为探针在免疫学的分子解剖中的应用 参与启动免疫反应或耐受的相互作用。 多个专业载体用于克隆和表达精选的 将制备DNA、限制性内切酶和放射性标记试剂，并 储存在核心设施中，供SCOR调查人员使用。 此外，我们将帮助个人的主要调查人员 进行一些更专门的实验的项目，包括 多种cDNA的克隆、表达、定点突变等 核酸操纵。最后，MRCL的工作人员将参与 稀有植物的提纯、分子鉴定及微测序 多肽产品(例如，参与转录的交易蛋白 控制胶原基因的表达)。取代蛋白质的MRCL 作为早期资助的SCOR的一部分而建立的化学核心，是 设想成为一个具有更大范围的微型化工设施，以充分 满足当前SCOR的需求。

We propose to establish a Molecular Resources Core Laboratory (MRCL), with the specific purpose of providing essential reagents, services and assistance with methodologies in support of the six subcomponents of the SCOR. This MRCL is designed as a centralized facility for the instrumentation, specialized molecular biology expertise, and the reagents in order to make it extremely cost effective and highly efficient. MRCL will help in the design and synthesis of oligonucleotides to be used as primers, probes and antisense reagents. We will also provide synthetic oligopeptides and site-specific variants of these peptides to be used as immunogens and as probes in the molecular dissection of the immunological interactions involved in the initiation of immune response or tolerance. A number of specialized vectors for cloning and expression of selected cDNAs, restriction enzymes, and radiolabeled reagents will be prepared and stored in the Core facility and made available to the SCOR investigators. In addition, we will help the principal investigators of the individual projects in carrying out some of the more specialized experiments involving cloning, expression, site-specific mutagenesis of various cDNAs, and other nucleic acid manipulations. Finally, MRCL personnel will be involved in purification, molecular characterization and microsequencing of the rare polypeptide products (e.g. transacting proteins involved in transcriptional control of collagen gene expression). The MRCL, which replaces the Protein Chemistry Core established as part of the earlier funded SCOR, is envisioned to be a microchemical facility with much greater scope to fully satisfy the needs of the current SCOR.