SOMATIC MUTATIONS AT THE GLYCOPHORIN A LOCUS IN RADIATION EXPOSED INDIVIDUALS

受辐射个体中血型糖蛋白 A 基因座的体细胞突变

• 批准号: 5209291
• 负责人: RICHARD G LANGLOIS
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5209291
• 关键词: Commonwealth of Independent States; DNA damage; erythrocytes; glycophorin; human genetic material tag; human subject; ionizing radiation; longitudinal human study; occupational hazard; questionnaires; radiation dosage; radiation genetics; statistics /biometry

There are two primary objectives for the glycophorin A-based somatic mutation analysis project. The first objective is to determine the magnitude and types of somatic mutational damage in liquidators exposed to radiation from the Chernobyl nuclear power accident as measured by the glycophorin A (GPA) assay. Analysis will be performed on blood samples from Russian liquidators with low-dose exposures and Russian controls, and will include measurements of the frequency of both hemizygous NO and homozygous NN variant erythrocytes. Measurements of the frequencies of somatic cell mutations at the GPA locus will be correlated with different indicators of genetic damage from other projects in the program project to provide a comprehensive analysis of genetic damage and potential health consequences in the liquidator population. The second objective is to utilize GPA data from the Chernobyl liquidator population to determine optimal strategies for detecting low-dose radiation exposures that may be applicable for future studies in other populations. Personal history data from questionnaires will be utilized to identify factors that contribute to inter-individual variability. Statistical procedures will be identified to adjust for confounding factors and facilitate differentiation between exposed and control populations. Results from the GPA assay will be compared with the results from other biodosimeters in the program project to determine the relative sensitivity and reliability of the different methods, and the power of the combined data for detecting low-dose radiation exposure in humans.

基于血型糖蛋白A的体细胞免疫有两个主要目的。 突变分析项目 第一个目标是确定 清理者暴露的体细胞突变损伤的程度和类型 切尔诺贝利核电站事故的辐射， 血型糖蛋白A（GPA）测定。 将对血液样本进行分析 来自俄罗斯清理者的低剂量暴露和俄罗斯控制， 并将包括半合子NO和 纯合子NN变异红细胞。 频率的测量 GPA基因座的体细胞突变将与不同的 该计划项目中其他项目的遗传损伤指标 提供一个全面的分析遗传损伤和潜在的 清算人的健康状况 第二个目标是利用来自切尔诺贝利清理人的GPA数据 人群确定检测低剂量的最佳策略 可能适用于其他国家未来研究的辐射暴露 人口。 将利用调查问卷中的个人历史数据 以确定导致个体间差异的因素。 将确定统计程序以调整混杂因素 因素和促进暴露和对照之间的差异 人口。 GPA测定的结果将与 项目中其他生物剂量计的结果，以确定 不同方法的相对灵敏度和可靠性，以及 用于检测低剂量辐射暴露的组合数据的功率， 人类