SOMATIC MUTATIONS AT THE GLYCOPHORIN A LOCUS IN RADIATION EXPOSED INDIVIDUALS

受辐射个体中血型糖蛋白 A 基因座的体细胞突变

• 批准号: 6102917
• 负责人: RICHARD G LANGLOIS
• 金额: $ 32.14万
• 依托单位: UNIVERSITY OF CALIF-LAWRNC LVRMR NAT LAB
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6102917
• 关键词: Commonwealth of Independent States; DNA damage; erythrocytes; glycophorin; human genetic material tag; human subject; ionizing radiation; longitudinal human study; occupational hazard; questionnaires; radiation dosage; radiation genetics; statistics /biometry

There are two primary objectives for the glycophorin A-based somatic mutation analysis project. The first objective is to determine the magnitude and types of somatic mutational damage in liquidators exposed to radiation from the Chernobyl nuclear power accident as measured by the glycophorin A (GPA) assay. Analysis will be performed on blood samples from Russian liquidators with low-dose exposures and Russian controls, and will include measurements of the frequency of both hemizygous NO and homozygous NN variant erythrocytes. Measurements of the frequencies of somatic cell mutations at the GPA locus will be correlated with different indicators of genetic damage from other projects in the program project to provide a comprehensive analysis of genetic damage and potential health consequences in the liquidator population. The second objective is to utilize GPA data from the Chernobyl liquidator population to determine optimal strategies for detecting low-dose radiation exposures that may be applicable for future studies in other populations. Personal history data from questionnaires will be utilized to identify factors that contribute to inter-individual variability. Statistical procedures will be identified to adjust for confounding factors and facilitate differentiation between exposed and control populations. Results from the GPA assay will be compared with the results from other biodosimeters in the program project to determine the relative sensitivity and reliability of the different methods, and the power of the combined data for detecting low-dose radiation exposure in humans.

糖蛋白为基础的体细胞有两个主要目的