MICA: High speed, high resolution imaging of excitable cell networks

MICA：可兴奋细胞网络的高速、高分辨率成像

• 批准号: MR/K015877/1
• 负责人: Tony Wilson
• 金额: $ 179.27万
• 依托单位: University of Oxford
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2013
• 资助国家: 英国
• 起止时间: 2013 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FK015877%2F1
• 关键词: MICA; speed; resolution; imaging; excitable

The heart and brain function through the fast propagation of electrical impulses. Heart arrhythmias and epileptic seizures are the results of massive electrical surges in these organs, but all cardiac and neurological dysfunction results ultimately from alterations in the patterns of biological electrical activity. Visualising activity in cardiac and neuronal tissue is a fundamental challenge for biomedicine, as each brief electrical signal propagates rapidly over large distances. For example, a single heartbeat involves choreographed waves of electrical activity pervading every cell in the organ. During the same time period, electrical signals in the nervous system can be used to detect the light reflected from an incoming cricket ball, calculate its trajectory, and send the motor commands to make the catch. Our proposal brings together two new techniques in optical engineering that will enable us to capture these rapid events in electrically excitable tissues.This proposal will create a high speed imaging facility, which will enable the wider application of these cutting-edge approaches to studying cardiac and neurological disease.

心脏和大脑通过电脉冲的快速传播来发挥作用。心律失常和癫痫发作是这些器官中大量电涌的结果，但所有心脏和神经功能障碍最终都是生物电活动模式改变的结果。可视化心脏和神经元组织的活动是生物医学的一个基本挑战，因为每个简短的电信号都能在很长的距离上快速传播。例如，一次心跳涉及到遍布器官中每个细胞的精心设计的电活动波。在同一时间段内，神经系统中的电信号可以用来检测从传入的板球反射的光，计算其轨迹，并发送运动指令来接球。我们的提案结合了光学工程中的两种新技术，使我们能够在电激发组织中捕获这些快速事件。该提案将创建一个高速成像设备，这将使这些尖端方法能够更广泛地应用于研究心脏和神经疾病。

项目成果

Hypertension-induced remodelling: on the interactions of cardiac risk factors.

• DOI: 10.1113/jp273043
• 发表时间: 2017-06-15
• 期刊: The Journal of physiology
• 作者: Tomek J;Bub G
• 通讯作者: Bub G

Optical control of excitation waves in cardiac tissue.

• DOI: 10.1038/nphoton.2015.196
• 发表时间: 2015-12
• 期刊: Nature photonics
• 影响因子: 35
• 作者: Burton RA;Klimas A;Ambrosi CM;Tomek J;Corbett A;Entcheva E;Bub G
• 通讯作者: Bub G

Macro-micro imaging of cardiac-neural circuits in co-cultures from normal and diseased hearts.

正常和患病心脏共培养物中心脏神经回路的宏观显微成像。

• DOI: 10.1113/jphysiol.2014.285460
• 发表时间: 2015
• 期刊: The Journal of physiology
• 作者: Bub G

Bringing the living brain into focus

让活的大脑成为焦点

• DOI: 10.1038/nphoton.2015.3
• 发表时间: 2015
• 期刊: Nature Photonics
• 影响因子: 35
• 作者: Corbett A

Quantifying distortions in two-photon remote focussing microscope images using a volumetric calibration specimen.

• DOI: 10.3389/fphys.2014.00384
• 发表时间: 2014
• 期刊: Frontiers in physiology
• 影响因子: 4
• 作者: Corbett AD;Burton RA;Bub G;Salter PS;Tuohy S;Booth MJ;Wilson T
• 通讯作者: Wilson T