ENDURANCE TRAINING EFFECTS ON GLUCOSE STORAGE PATHWAYS IN DIABETIC ANIMAL

耐力训练对糖尿病动物葡萄糖储存途径的影响

• 批准号: 5211869
• 负责人: HANS M GUNDERSON
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5211869
• 关键词: blood glucose; carbohydrate balance; disease /disorder model; exercise; glucose metabolism; glucose transport; glycogenolysis; hydrolysis; laboratory rat; noninsulin dependent diabetes mellitus; nuclear magnetic resonance spectroscopy; phosphorylation; respiratory gas analyzer; scintillation counter; ultraviolet spectrometry

The maintenance of a normal steady concentration of glucose in the blood is necessary for good health. The diabetes occurs when a person can no longer adequately control the level of blood sugar, and it rises out of the normal range. Diabetes is a major health problem in the United States, affecting one person in twenty (5% of the population). Among the large population of Native Americans in Arizona, the frequency of diabetes is even more significant, occurring four to eight times more often than the national average. Minority students selected for this project will almost certainly have had a direct personal experiences with diabetes. Several laboratory studies have shown that endurance exercise improves an individual's regulation of blood glucose levels. The biochemical reason for this training effect is currently being investigated. An important factor is the body's ability to store glycogen, which can be made either directly or indirectly in the liver. The exercise trained individual may be capable of controlling blood glucose levels by more efficient control of glycogen storage. The specific aims of this project are to continue our studies by using diabetic animal models to determine the relative contributions of the direct and indirect pathways of glycogen formation, activities of key enzymes, amount of muscles glucose transporters, the sources and levels of blood glucose, and how these parameters are affected by enduring training. The minority biomedical students who will participate in this project will actively use biochemical research principles and techniques. They will train the animals and do biochemical assays. They will learn to use in a safe manner instruments such as a uv-visible spectrophotometer, blood analyzers, electrophoresis apparatus, a scintillation counter, an NMR spectrometer, CO2 and O2 analyzers, and chromatographic equipment. These students will also be actively involved in analyzing and interpreting data, designing experiments and in disseminating the conclusions through presentations at meetings and publications in the biomedical literature.

维持血液中正常稳定的葡萄糖浓度 对于身体健康是必要的。 当一个人无法 更长时间地充分控制血糖水平，并且它会上升 正常范围。 糖尿病是美国的一个主要健康问题 州，影响二十分之一的人（占人口的 5%）。 其中 亚利桑那州有大量美洲原住民， 糖尿病更为严重，发生率是其四到八倍 往往高于全国平均水平。 本次选拔的少数民族学生 项目几乎肯定会有直接的个人经历 糖尿病。 多项实验室研究表明耐力运动可以改善 个人对血糖水平的调节。 生化 目前正在调查造成这种训练效果的原因。 一个 重要因素是身体储存糖原的能力，可以 直接或间接在肝脏中产生。 所训练的运动 个体可能能够通过更多的方式控制血糖水平 有效控制糖原储存。 该项目的具体目标 将通过使用糖尿病动物模型来继续我们的研究以确定 直接和间接途径的相对贡献 糖原形成、关键酶的活性、肌肉葡萄糖量 转运蛋白、血糖的来源和水平，以及它们如何 参数会受到持久训练的影响。 参与本项目的少数民族生物医学生 将积极运用生化研究原理和技术。 他们 将训练动物并进行生化分析。 他们将学习使用 以安全的方式使用仪器，例如紫外可见分光光度计， 血液分析仪、电泳仪、闪烁计数器、 核磁共振波谱仪、CO2 和 O2 分析仪以及色谱设备。 这些学生也将积极参与分析和 解释数据、设计实验和传播 通过会议上的发言和出版物得出的结论 生物医学文献。