ENDURANCE TRAINING EFFECTS ON GLUCOSE STORAGE PATHWAYS IN DIABETIC ANIMAL

耐力训练对糖尿病动物葡萄糖储存途径的影响

• 批准号: 6240245
• 负责人: HANS M GUNDERSON
• 金额: $ 4.53万
• 依托单位: NORTHERN ARIZONA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-03-01 至 1998-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6240245
• 关键词: blood glucose; carbohydrate balance; disease /disorder model; exercise; glucose metabolism; glucose transport; glycogenolysis; hydrolysis; laboratory rat; noninsulin dependent diabetes mellitus; nuclear magnetic resonance spectroscopy; phosphorylation; respiratory gas analyzer; scintillation counter; ultraviolet spectrometry

The maintenance of a normal steady concentration of glucose in the blood is necessary for good health. The diabetes occurs when a person can no longer adequately control the level of blood sugar, and it rises out of the normal range. Diabetes is a major health problem in the United States, affecting one person in twenty (5% of the population). Among the large population of Native Americans in Arizona, the frequency of diabetes is even more significant, occurring four to eight times more often than the national average. Minority students selected for this project will almost certainly have had a direct personal experiences with diabetes. Several laboratory studies have shown that endurance exercise improves an individual's regulation of blood glucose levels. The biochemical reason for this training effect is currently being investigated. An important factor is the body's ability to store glycogen, which can be made either directly or indirectly in the liver. The exercise trained individual may be capable of controlling blood glucose levels by more efficient control of glycogen storage. The specific aims of this project are to continue our studies by using diabetic animal models to determine the relative contributions of the direct and indirect pathways of glycogen formation, activities of key enzymes, amount of muscles glucose transporters, the sources and levels of blood glucose, and how these parameters are affected by enduring training. The minority biomedical students who will participate in this project will actively use biochemical research principles and techniques. They will train the animals and do biochemical assays. They will learn to use in a safe manner instruments such as a uv-visible spectrophotometer, blood analyzers, electrophoresis apparatus, a scintillation counter, an NMR spectrometer, CO2 and O2 analyzers, and chromatographic equipment. These students will also be actively involved in analyzing and interpreting data, designing experiments and in disseminating the conclusions through presentations at meetings and publications in the biomedical literature.

维持血液中葡萄糖的正常稳定浓度 是身体健康所必需的。 糖尿病发生时，一个人不能 更长的时间充分控制血糖水平，它上升了， 正常范围 糖尿病是美国的一个主要健康问题。 影响到二十分之一的人（占人口的5%）。 中 亚利桑那州的大量美洲原住民， 糖尿病的发病率更高，是其他疾病的4到8倍。 往往高于全国平均水平。 少数民族学生被选中 几乎可以肯定的是，项目将有直接的个人经验， 糖尿病 几项实验室研究表明，耐力运动可以改善 个体对血糖水平的调节。 生化 目前正在调查这种培训效果的原因。 一个 一个重要的因素是身体储存糖原的能力， 直接或间接在肝脏中产生。 演习训练了 个体可能能够通过更高血糖水平来控制血糖水平 有效控制糖原储存。 该项目的具体目标 继续我们的研究，使用糖尿病动物模型， 直接和间接途径的相对贡献 糖原形成，关键酶活性，肌肉葡萄糖含量 转运蛋白，血糖的来源和水平，以及这些 参数受持久训练的影响。 少数民族生物医学专业的学生将参加这个项目 将积极运用生物化学研究原理和技术。 他们 将训练动物并进行生化分析。 他们将学会使用 以安全的方式使用诸如紫外可见分光光度计的仪器， 血液分析仪、电泳仪、闪烁计数器、 NMR光谱仪、CO2和O2分析仪和色谱设备。 这些学生也将积极参与分析和 解释数据、设计实验和传播 通过在会议上的发言和在联合国出版物 生物医学文献