Molecular and cellular characterisation of an important new human DNA repair disorder

一种重要的新型人类 DNA 修复障碍的分子和细胞特征

基本信息

  • 批准号:
    MR/L006812/1
  • 负责人:
  • 金额:
    $ 51.94万
  • 依托单位:
  • 依托单位国家:
    英国
  • 项目类别:
    Research Grant
  • 财政年份:
    2014
  • 资助国家:
    英国
  • 起止时间:
    2014 至 无数据
  • 项目状态:
    已结题

项目摘要

There are a group of rare inherited genetic disorders that are caused by the mutation of genes that normally act to protect our genes from damage. The chemical that comprises our genetic information, DNA, is susceptible to damage from a wide variety of sources, including environmental exposure or cellular metabolism. Individuals that inherit these DNA damage sensitivity disorders have distinctive combinations of symptoms, resulting from the abnormal behaviour of the mutated genes. These symptoms can include premature ageing, growth defects, neurodegeneration, sunlight or X-ray sensitivity and abnormal skin pigmentation. We recently identified a new syndrome in the Ohio Amish community that is distinct from, but appears related to, known DNA damage sensitivity conditions such as the disorders known as XP (xeroderma pigmentosum), CS (Cockayne's syndrome) or AT (ataxia telangiectasia). The affected individuals have a unique combination of symptoms that led us to hypothesize that a previously unreported mutation must be causing the syndrome. Using state of the art genetic mapping techniques and genome sequencing we have identified the gene and the mutation responsible for causing this disorder.We now need to understand what this particular genetic change does in order to cause the symptoms of the affected individuals. This is important for three reasons. Firstly, if we understand better the problems faced by the cells of affected individuals we should be able to manage their condition more effectively. Secondly, understanding how cells behave when this gene is altered will give us important information about how the unmutated gene normally functions. This is crucial because all our cells are constantly facing DNA damage, and failure to deal with this damage properly can lead to mutations that can result in cancer, ageing and neurodegeneration. A knowledge of the processes that cells normally use to detect, repair or tolerate DNA damage can therefore be hugely valuable in understanding how cancer and neurodegeneration are normally prevented, and what has to go wrong in order for them to occur. Finally, in the clinical setting we use chemicals and treatments that induce DNA damage as chemo- and radio-therapies for many different type of cancer. A better understanding of how our cells tolerate DNA damage, and how specific cells can be made more susceptible to these treatments, can help research aimed at improving therapies and developing novel treatments for malignant disease. Through a longstanding community collaborative program we have unique access to cellular material derived from skin biopsies and blood samples from the affected individuals and unaffected family members. Our proposal is to test these cells in the laboratory to find out how their behaviour differs from normal cells, for example when treated with DNA damaging agents. We will use state-of the-art molecular biology, microscopy and biochemical approaches to uncover why the mutated gene causes these altered cell behaviours. We will throughout the project keep in close communication with the local clinicians to ensure that our findings are used to optimise the treatment of the affected individuals, and we will also make sure that other researchers are informed about our results (via publication, networking and presentation at conferences), so that our research can be translated to generate maximum benefit for human health.
有一组罕见的遗传性遗传疾病是由基因突变引起的,这些基因通常起保护我们的基因免受损害的作用。构成我们遗传信息的化学物质DNA容易受到各种来源的损害,包括环境暴露或细胞代谢。遗传这些DNA损伤敏感性疾病的个体具有独特的症状组合,这是由突变基因的异常行为引起的。这些症状可能包括过早衰老、生长缺陷、神经退行性变、阳光或X射线敏感以及异常皮肤色素沉着。我们最近在俄亥俄州阿米什人社区发现了一种新的综合征,它与已知的DNA损伤敏感性疾病不同,但似乎与之相关,如被称为XP(着色性干皮病)、CS(Cockayne综合征)或AT(共济失调毛细血管扩张症)的疾病。受影响的个体具有独特的症状组合,这使我们假设以前未报告的突变必须引起该综合征。使用最先进的基因图谱技术和基因组测序,我们已经确定了导致这种疾病的基因和突变。我们现在需要了解这种特殊的基因变化是如何导致受影响个体的症状的。这一点很重要,原因有三。首先,如果我们更好地了解受影响个体的细胞所面临的问题,我们应该能够更有效地管理他们的病情。其次,了解当这个基因被改变时细胞的行为将为我们提供关于未突变基因正常功能的重要信息。这是至关重要的,因为我们所有的细胞都不断面临DNA损伤,如果不能正确处理这种损伤,可能会导致突变,导致癌症,衰老和神经退行性变。因此,了解细胞通常用于检测、修复或耐受DNA损伤的过程,对于理解癌症和神经退行性变通常是如何预防的,以及它们发生的原因是什么,都是非常有价值的。最后,在临床环境中,我们使用诱导DNA损伤的化学品和治疗方法作为许多不同类型癌症的化疗和放疗。更好地了解我们的细胞如何耐受DNA损伤,以及如何使特定细胞对这些治疗更敏感,可以帮助旨在改善治疗方法和开发恶性疾病新疗法的研究。通过一个长期的社区合作计划,我们有独特的机会获得来自受影响的个人和未受影响的家庭成员的皮肤活检和血液样本的细胞材料。我们的建议是在实验室中测试这些细胞,以找出它们的行为与正常细胞的不同之处,例如当用DNA损伤剂处理时。我们将使用最先进的分子生物学,显微镜和生物化学方法来揭示为什么突变的基因会导致这些改变的细胞行为。我们将在整个项目中与当地临床医生保持密切沟通,以确保我们的研究结果用于优化受影响个体的治疗,我们还将确保其他研究人员了解我们的结果(通过出版物,网络和会议上的演示),以便我们的研究可以转化为人类健康的最大利益。

项目成果

期刊论文数量(4)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
A fluorescent bimolecular complementation screen reveals MAF1, RNF7 and SETD3 as PCNA-associated proteins in human cells.
  • DOI:
    10.1080/15384101.2015.1053667
  • 发表时间:
    2015-08-03
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Cooper SE;Hodimont E;Green CM
  • 通讯作者:
    Green CM
PCNA dependent cellular activities tolerate dramatic perturbations in PCNA client interactions.
PCNA依赖性细胞活性在PCNA客户端相互作用中耐受剧烈扰动。
  • DOI:
    10.1016/j.dnarep.2016.12.003
  • 发表时间:
    2017-02
  • 期刊:
  • 影响因子:
    3.8
  • 作者:
    Wilson RH;Biasutto AJ;Wang L;Fischer R;Baple EL;Crosby AH;Mancini EJ;Green CM
  • 通讯作者:
    Green CM
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Catherine Green其他文献

Measuring Reasoning in Paranoia: Development of the Fast and Slow Thinking Questionnaire
测量偏执狂的推理:快速和慢速思维问卷的开发
  • DOI:
  • 发表时间:
    2020
  • 期刊:
  • 影响因子:
    0
  • 作者:
    A. Hardy;E. Tolmeijer;V. Edwards;T. Ward;D. Freeman;R. Emsley;Catherine Green;M. Rus;K. Greenwood;P. Bebbington;E. Kuipers;D. Fowler;Catarina Sacadura;Nicola Collett;A. McGourty;G. Dunn;P. Garety
  • 通讯作者:
    P. Garety
Sharpening the focus on ophthalmology teaching: has anything changed?
加强对眼科教学的关注:有什么变化吗?
Intrusive mental imagery in patients with persecutory delusions
  • DOI:
    10.1016/j.brat.2012.10.002
  • 发表时间:
    2013-01-01
  • 期刊:
  • 影响因子:
  • 作者:
    Katja Schulze;Daniel Freeman;Catherine Green;Elizabeth Kuipers
  • 通讯作者:
    Elizabeth Kuipers
Bilateral consecutive Xen gel stent surgery during pregnancy for uncontrolled early-onset primary open angle glaucoma
  • DOI:
    10.1016/j.ajoc.2019.100510
  • 发表时间:
    2019-09-01
  • 期刊:
  • 影响因子:
  • 作者:
    Tzukit Zehavi-Dorin;Evan Heinecke;Shivram Nadkarni;Catherine Green;Christine Chen;Yu Xiang George Kong
  • 通讯作者:
    Yu Xiang George Kong

Catherine Green的其他文献

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