How to assemble the cardiovascular system: instructions from the genome

如何组装心血管系统：来自基因组的指令

• 批准号: MR/L009986/1
• 负责人: Nicoletta Bobola
• 金额: $ 85.63万
• 依托单位: University of Manchester
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2014
• 资助国家: 英国
• 起止时间: 2014 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FL009986%2F1
• 关键词: How; assemble; cardiovascular; system; instructions

Cardiovascular disease is the leading cause of death worldwide. Inherited DNA sequence variants play a role in conferring risk for disease. Our knowledge of the genetic loci associated with risk of cardiovascular disease in humans has been greatly expanded by recent technological advancements. However, only a fraction of the genetic risk factors have been identified and characterized so far. A major problem is that only a small fraction of the genome, the one occupied by protein-coding genes, is understood in terms of its function. Therefore, we can only predict the harmful effects of genetic variations occurring within this fraction. However, the vast majority of sequence variants associated to disease reside elsewhere in the genome. Thus, it is crucial that the non-coding functional genome (regulatory genome) be discovered and characterized. This project will use state-of-the-art technologies to identify the functional, non-coding human genome that controls formation of the outflow tract of the heart and the great vessels. We will then link the identified human regulatory information to known genetic variations associated to cardiovascular disease risk, and to available data from our collaborators on congenital heart disease (CHD). Our results will be stored into a searchable, open-access web application to facilitate their clinical translation. We expect that the results of this project will lead to the discovery of genetic variants associated to congenital heart disease and clinical phenotypes disease risk, and will eventually expand diagnostic and therapeutic capacities.

心血管疾病是全世界死亡的主要原因。遗传的DNA序列变异在赋予疾病风险方面发挥作用。最近的技术进步极大地扩展了我们对与人类心血管疾病风险相关的遗传位点的了解。然而，到目前为止，只有一小部分遗传风险因素被确定和表征。一个主要的问题是，只有一小部分基因组，即蛋白质编码基因所占的基因组，才能从功能上得到理解。因此，我们只能预测在这一部分中发生的遗传变异的有害影响。然而，绝大多数与疾病相关的序列变异存在于基因组的其他地方。因此，发现和表征非编码功能基因组（调控基因组）至关重要。该项目将使用最先进的技术来识别控制心脏流出道和大血管形成的功能性非编码人类基因组。然后，我们将识别出的人类监管信息与已知的与心血管疾病风险相关的遗传变异以及我们的合作者在先天性心脏病（CHD）方面的可用数据联系起来。我们的结果将被存储到一个可搜索的，开放访问的网络应用程序，以促进其临床翻译。我们预计，该项目的结果将导致发现与先天性心脏病和临床表型疾病风险相关的遗传变异，并最终扩大诊断和治疗能力。

项目成果

From DNA binding to transcriptional activation: Is the TALE complete?

• DOI: 10.1083/jcb.201706113
• 发表时间: 2017-09-04
• 期刊: The Journal of cell biology
• 作者: Bobola N

A tissue-specific, Gata6-driven transcriptional program instructs remodeling of the mature arterial tree.

• DOI: 10.7554/elife.31362
• 发表时间: 2017-09-27
• 期刊: eLife
• 影响因子: 7.7
• 作者: Losa M;Latorre V;Andrabi M;Ladam F;Sagerström C;Novoa A;Zarrineh P;Bridoux L;Hanley NA;Mallo M;Bobola N
• 通讯作者: Bobola N

HOX paralogs selectively convert binding of ubiquitous transcription factors into tissue-specific patterns of enhancer activation.

• DOI: 10.1371/journal.pgen.1009162
• 发表时间: 2020-12
• 期刊: PLoS genetics
• 影响因子: 4.5
• 作者: Bridoux L;Zarrineh P;Mallen J;Phuycharoen M;Latorre V;Ladam F;Losa M;Baker SM;Sagerstrom C;Mace KA;Rattray M;Bobola N
• 通讯作者: Bobola N

Laser Capture and Deep Sequencing Reveals the Transcriptomic Programmes Regulating the Onset of Pancreas and Liver Differentiation in Human Embryos.

• DOI: 10.1016/j.stemcr.2017.09.018
• 发表时间: 2017-11-14
• 期刊: Stem cell reports
• 影响因子: 5.9
• 作者: Jennings RE;Berry AA;Gerrard DT;Wearne SJ;Strutt J;Withey S;Chhatriwala M;Piper Hanley K;Vallier L;Bobola N;Hanley NA
• 通讯作者: Hanley NA

Hoxa2 selectively enhances Meis binding to change a branchial arch ground state.

• DOI: 10.1016/j.devcel.2014.12.024
• 发表时间: 2015-02-09
• 期刊: DEVELOPMENTAL CELL
• 影响因子: 11.8
• 作者: Amin, Shilu;Donaldson, Ian J.;Zannino, Denise A.;Hensman, James;Rattray, Magnus;Losa, Marta;Spitz, Francois;Ladam, Franck;Sagerstroem, Charles;Bobola, Nicoletta
• 通讯作者: Bobola, Nicoletta