A cell atlas of the human outflow tract of the heart

影响心脏流出道的缺陷占所有先天性心脏病病例的三分之一。它们通常导致新生儿的血液循环和/或氧合异常，并在出生后不久需要手术矫正。OFT的正确发育和功能取决于不同细胞类型之间的相互作用。这些细胞类型包含相同的遗传指令，但产生不同的蛋白质，因为它们表达不同的基因。我们将使用新技术，这将使我们能够表征单细胞表达的基因，将人类OFT组织解构为其组成细胞类型，并追踪胚胎发育过程中细胞的不同起源。总的来说，这些结果将为健康和患病细胞状态是如何建立的提供新的见解，并对疾病诊断、个性化和再生医学产生影响。

Defects affecting the outflow tract (OFT) of the heart represent a third of all congenital heart disease cases. They often result in abnormal blood circulation and/or oxygenation in the neonate and require surgical correction shortly after birth. Correct development and function of the OFT depend on the interaction between different cell types. These cell types contain the same genetic instructions but produce different proteins because they express different genes. We will use new technologies, which will allow us to characterize single-cell expressed genes, to deconstruct the human OFT tissue into its constituent cell types and trace the diverse origins of the cells during embryonic development. Collectively, these results will provide new insight into how healthy and diseased cell states are established, with knock-on implications in disease diagnosis and personalized and regenerative medicine.