CORTICAL BLOOD FLOW CHANGES PROVOKED BY COGNITIVE TASKS IN NORMAL VOLUNTEERS

正常志愿者认知任务引起的皮质血流变化

• 批准号: 2353442
• 金额: --
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2353442
• 关键词: clinical research; cognition; drug administration rate /duration; human subject; single photon emission computed tomography; volunteer

The purpose of the study is to investigate SPECT blood flow measurement of cortical activation when the ligand is administered as a steady infusion over a 5-6 minute period of time, rather than a bolus dose at one time. In addition, the Wisconsin Card Sorting Test, used as the activating task, was first taught to subjects so that the activation effect could be ascribed as due to the cognitive actions of performing the test rather than learning the test. The methodology for the performance of the Wisconsin Card Sorting Test (WCST) was developed for computer administration, providing the most reliable means of task performance for the subjects. Subjects were taught the WCST task prior to SPECT imaging. The logic for this was to remove sources of variance in WCST performance due to learning effects, rather than continuous performance of the cognitive functions needed to perform the WCST. WCST performance was recorded, and all subjects performed very well on it. Details of administration of the SPECT ligand using a perfusion pump were established. This entailed determination of fluid volumes within the IV tubing, and calculation of flow rates. A perfusion rate was used to provide 5-6 minutes of constant administration of SPECT ligand to the subject. The perfusion was begun shortly after subjects began the task (either control task or WCST activation task), and ended before subjects completed the task. Five normal subjects have been assessed with two SPECT scans each, the baseline control task and the activation task. All subjects performed well on the WCST activation task. SPECT image data has been analyzed. Statistical Parametric Mapping (SPM) has been used to normalize images with respect to standard neuroanatomical references points. This involves identification of landmarks, and rotation and translation of the image data sets. After normalization, the baseline control task images have been digitally subtracted from the WCST activation task. These differences have been pooled across all five subjects. Activation was larger in the right frontal lobe, and inferior parietal, superior temporal, and orbito-medial frontal areas showed significant activation. Inactivation was greatest on the right side in the area of the hippocampus, and somewhat less pronounced in the left hippocampus. These findings are similar to results published by other groups for WCST activation, but differ in showing involvement of orbito-medial frontal areas.

本研究的目的是研究 SPECT 血流测量 当配体作为稳定剂施用时的皮质激活 输注时间为 5-6 分钟，而不是一次推注剂量 一度。 此外，威斯康星卡片分类测试，用作 激活任务，首先教给受试者，以便激活 效果可以归因于执行的认知行为 测试而不是学习测试。 威斯康星卡片分类测试的实施方法 (WCST) 是为计算机管理而开发的，提供了最 受试者执行任务的可靠手段。 受试者是 在 SPECT 成像之前教授 WCST 任务。 这样做的逻辑是 消除因学习而导致 WCST 表现差异的来源 效果，而不是认知功能的持续表现 需要执行 WCST。 WCST 表现已被记录，并且所有 受试者在这方面表现得很好。 使用灌注泵施用 SPECT 配体的详细信息 成立了。 这需要确定内部的液体体积 IV 管和流量计算。 使用灌注速率 提供 5-6 分钟的 SPECT 配体持续给药 主题。 受试者开始任务后不久开始灌注 （控制任务或 WCST 激活任务），并在受试者之前结束 完成了任务。 五名正常受试者每人接受两次 SPECT 扫描评估， 基线控制任务和激活任务。 所有科目均已完成 WCST 激活任务进展顺利。 SPECT 图像数据已被分析。 统计参数映射 （SPM）已用于根据标准对图像进行归一化 神经解剖学参考点。 这涉及到识别 地标以及图像数据集的旋转和平移。 后 归一化，基线控制任务图像已被数字化 从 WCST 激活任务中减去。 这些差异已被 汇总所有五个科目。 右额叶和顶下叶的激活较大， 颞上区和眶内侧额区显示出显着的 激活。 右侧区域的失活最为严重 海马体，左侧海马体不太明显。 这些发现与其他小组为 WCST 发表的结果相似 激活，但在显示眶内侧额叶的参与方面有所不同 地区。