STRUCTURE/FUNCTION OF IDENTIFIED SYNAPSES IN THE CNS

中枢神经系统中已识别突触的结构/功能

• 批准号: 2883588
• 负责人: DAVID K SELIG
• 金额: $ 3.84万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2883588
• 关键词: AMPA receptors; central nervous system; electron microscopy; electrophysiology; hippocampus; laboratory rat; long term potentiation; neuroanatomy; neurophysiology; neurotransmitter transport; pyramidal cells; synapses; synaptic vesicles; voltage /patch clamp

This study of synaptically connected CA3 pyramidal cells will attempt to answer the following questions: 1. Are pairs of CA3 pyramidal cells connected by a single synapse? 2. Is the distribution of synaptic responses skewed? 3. Does the size of the presynaptic bouton correlate with synaptic strength? 4. Does the size of the postsynaptic density correlate with the quantal size? 5. Does the number of docked synaptic vesicles correlate with the probability of release? These questions will be answered by recording synaptic responses between pairs of CA3 pyramidal cells while filling those cells with a cell impermeant tracer. The neuronal processes of the two cells will be reconstructed, and electron microscopy will be used to determine the ultrastructure of all synapses involved in the synaptic response. In pairs involving only a single synapse, the ultrastructure and synaptic responses will be compared. The results of this study will provide important information about the basic functional and structural properties of individual excitatory synapses in the hippocampus and will provide a solid foundation, that has been lacking, for future studies into the electrophysiological mechanisms and ultrastructural underpinnings of long-term potentiation.

这项突触连接的CA3锥体细胞的研究将尝试 回答以下问题：1.成对的CA3锥体细胞 由单个突触连接？2.突触的分布 反应是否偏斜？3.突触前突触的大小是否与 突触强度？4.突触后密度的大小 与量子大小相关吗？5.停靠的突触数量 囊泡与释放的可能性有关吗？这些问题 将通过记录CA3对之间的突触反应来回答 锥体细胞，同时用不必要的细胞填充这些细胞 追踪剂。这两个细胞的神经元突起将被重建， 并将使用电子显微镜来确定超微结构 在所有参与突触反应的突触中。成双成对，涉及 只有一个突触，超微结构和突触反应 被比较。这项研究的结果将提供重要的 有关的基本功能和结构属性的信息 单独的兴奋性突触在海马区，并将提供 一直缺乏的坚实基础，为未来对 电生理机制及超微结构基础 长时程增强。