STRUCTURE/FUNCTION OF IDENTIFIED SYNAPSES IN THE CNS

这项研究的突触连接CA3锥体细胞将尝试 回答以下问题：1.是成对的CA3锥体细胞 由一个突触连接在一起2.是突触的分布 反应偏？3.突触前终扣的大小是否与 突触的强度？4.突触后密度的大小 与量子尺寸有关吗5.停靠的突触数量 囊泡与释放的可能性相关吗？这些问题 将通过记录CA3对之间的突触反应来回答。 锥体细胞，同时用细胞不渗透物填充这些细胞 追踪器两个细胞的神经突起将被重建， 电镜观察其超微结构 所有参与突触反应的突触。两人一组， 只有一个突触，超微结构和突触反应将 被比较。这项研究的结果将提供重要的 关于基本功能和结构特性的信息 海马体中的单个兴奋性突触，并将提供 为今后的研究奠定了坚实的基础， 电生理机制和超微结构基础 长时程增强

This study of synaptically connected CA3 pyramidal cells will attempt to answer the following questions: 1. Are pairs of CA3 pyramidal cells connected by a single synapse? 2. Is the distribution of synaptic responses skewed? 3. Does the size of the presynaptic bouton correlate with synaptic strength? 4. Does the size of the postsynaptic density correlate with the quantal size? 5. Does the number of docked synaptic vesicles correlate with the probability of release? These questions will be answered by recording synaptic responses between pairs of CA3 pyramidal cells while filling those cells with a cell impermeant tracer. The neuronal processes of the two cells will be reconstructed, and electron microscopy will be used to determine the ultrastructure of all synapses involved in the synaptic response. In pairs involving only a single synapse, the ultrastructure and synaptic responses will be compared. The results of this study will provide important information about the basic functional and structural properties of individual excitatory synapses in the hippocampus and will provide a solid foundation, that has been lacking, for future studies into the electrophysiological mechanisms and ultrastructural underpinnings of long-term potentiation.