FIBER OPTIC SENSOR FOR IN VIVO MONITORING OF L-GLUTAMATE

描述：(申请者摘要)L-谷氨酸是关键之一 神经递质，在突触可塑性中起主要作用。长期的 兴奋性突触神经传递的突触效能变化 被认为是学习和记忆的基础。 现有的测定L-谷氨酸浓度的方法包括微透析， 随后采用基于酶的微电极检测，高性能 色谱，或毛细管电泳法。这些方法存在一个问题) 量化困难，b)时间分辨率差(&gt；2分钟)，以及c) 来自L的干扰-抗坏血酸；由于缺乏更好的产品，它们已被使用 感知机制。 智能光学系统公司(IOS)建议开发一种实时 用于L-谷氨酸浓度在体监测的荧光电极传感器 在大鼠脑细胞中。建议的设备将消除几个瓶颈 影响现有方法，并将极大地促进大脑 研究。为了实现这一目标，我们将开发荧光探针 L-基于光致电子转移的谷氨酸。这些荧光探针 将被固定在多个微米尺寸的光纤探头上。检测和 将开发成像系统来监测动态和空间变化 发生在L-谷氨酸浓度的大鼠脑细胞中。 建议的商业应用：不可用

DESCRIPTION: (Applicant's abstract) L-glutamate is one of the key neurotransmitters and plays a primary role in synaptic plasticity. Long-term changes in synaptic efficacy of excitatory synaptic neurotransmission are considered to be the basis for learning and memory. Existing methods to measure L-glutamate concentration involve microdialysis, followed by detection using enzyme-based microelectrodes, high-performance chromatography, or capillary electrophoresis. These methods suffer from a) difficulties in quantitation, b) poor temporal resolution (>2 minutes), and c) interference from L-ascorbate; they have been used due to lack of better sensing mechanisms. Intelligent Optical Systems, Inc. (IOS) proposes to develop a real-time fluorescent optrode sensor for in vivo monitoring of L-glutamate concentration in rat brain cells. The proposed device will eliminate several bottlenecks affecting existing methodologies, and will substantially advance brain research. To achieve this goal we will develop fluorescent probes for L-glutamate based on photoinduced electron transfer. These fluorescent probes will be immobilized on multiple micron-sized fiber optic probes. Detection and imaging systems will be developed to monitor the dynamic and spatial changes occurring in L-glutamate concentrations in rat brain cells. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE