FIBER OPTIC SENSOR FOR IN VIVO MONITORING OF L-GLUTAMATE

用于 L-谷氨酸体内监测的光纤传感器

• 批准号: 2786218
• 负责人: Srivatsa Venkatasubbarao
• 金额: $ 10万
• 依托单位: INTELLIGENT OPTICAL SYSTEMS, INC.
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-06-07 至 2000-06-06
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2786218
• 关键词: biomedical equipment development; chemical synthesis; electron transport; fiber optics; fluorescence; fluorescent dye /probe; glutamates; light microscopy; monitoring device; neurochemistry

DESCRIPTION: (Applicant's abstract) L-glutamate is one of the key neurotransmitters and plays a primary role in synaptic plasticity. Long-term changes in synaptic efficacy of excitatory synaptic neurotransmission are considered to be the basis for learning and memory. Existing methods to measure L-glutamate concentration involve microdialysis, followed by detection using enzyme-based microelectrodes, high-performance chromatography, or capillary electrophoresis. These methods suffer from a) difficulties in quantitation, b) poor temporal resolution (>2 minutes), and c) interference from L-ascorbate; they have been used due to lack of better sensing mechanisms. Intelligent Optical Systems, Inc. (IOS) proposes to develop a real-time fluorescent optrode sensor for in vivo monitoring of L-glutamate concentration in rat brain cells. The proposed device will eliminate several bottlenecks affecting existing methodologies, and will substantially advance brain research. To achieve this goal we will develop fluorescent probes for L-glutamate based on photoinduced electron transfer. These fluorescent probes will be immobilized on multiple micron-sized fiber optic probes. Detection and imaging systems will be developed to monitor the dynamic and spatial changes occurring in L-glutamate concentrations in rat brain cells. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

描述：（申请人摘要）l -谷氨酸是关键之一