课题基金 / 基金详情

NITRIC OXIDE-MEDIATED PROTEIN MODIFICATION IN THE LUNG

NITRIC OXIDE-MEDIATED PROTEIN MODIFICATION IN THE LUNG
一氧化氮介导的肺部蛋白质修饰
批准号:
6030791
负责人:
CARROLL E CROSS
金额:
$18.23万
依托单位国家:
美国
项目类别:
财政年份:
1997
资助国家:
美国
项目状态:
已结题
起止时间:
1997-07-23 至 2001-06-30

项目摘要

项目成果

CARROLL E CROSS的其他基金

相似基金

相关文献

中文摘要
翻译
描述(根据申请人的摘要和具体目的改编)
英文摘要
DESCRIPTION (Adapted from applicant's abstract and specific aims): This project represents a multidisciplinary approach overlapping chemistry, biochemistry, and cell biology, to study the effect of endogenously and exogenously formed nitric oxide (NO) and other reactive nitrogen species (RNS) upon protein modification in the lung. The overall hypothesis is the glutathione S-transferases are preferential targets for tyrosine nitration and chlorination in the inflamed lung, and hence, may serve as appropriate dosimeters for the production of reactive species in vivo. In addition, nitration and/or chlorination of functional tyrosine residues in GSTs may result in enzyme inactivation and weakened cellular defenses. The specific aims are: 1) to refine existing analytical methods for the simultaneous and site-specific measurement of multiple protein modifications (tyrosine nitration and chlorination) in glutathione S-transferases using electrospray ionization tandem mass spectrometry; 2) to ascertain whether chemical- and neutrophil-mediated nitration and chlorination of conserved active-site tyrosyl residues in purified rat and human glutathione S-transferases inhibits their activity in vitro, and to determine which isozymes are most susceptible to modification and inactivation; 3) to determine whether reactive nitrogen species or stimulated human neutrophils induce nitration and/or chlorination of active-site tyrosines in glutathione S-transferase isozymes in cultured human tracheobronchial epithelial cells (HTBECs), and to determine whether this results in decreased activity of this cellular defense system; and 4) to determine whether nitrated and chlorinated glutathione S-transferases are present in lung tissue from cigarette smokers and patients with inflammatory lung diseases, and to correlate the extent to which these modifications impair the function of this critical cellular defense system.
期刊论文(4)
专著(0)
科研奖励(0)
会议论文
Reactive nitrogen species and tyrosine nitration in the respiratory tract: epiphenomena or a pathobiologic mechanism of disease?
呼吸道中的活性氮和酪氨酸硝化:副现象还是疾病的病理生物学机制?
DOI: 10.1164/ajrccm.160.1.9807044
发表时间: 1999
期刊: American journal of respiratory and critical care medicine.
影响因子: --
作者: [vanderVliet,A, Eiserich,JP, Shigenaga,MK, Cross,CE]
通讯作者: Cross,CE
Formation of S-nitrosothiols via direct nucleophilic nitrosation of thiols by peroxynitrite with elimination of hydrogen peroxide.
通过过氧亚硝酸盐对硫醇进行直接亲核亚硝化并消除过氧化氢,形成 S-亚硝基硫醇。
DOI: 10.1074/jbc.273.46.30255
发表时间: 1998
期刊: The Journal of biological chemistry
影响因子: --
作者: [vanderVliet,A, Hoen,PA, Wong,PS, Bast,A, Cross,CE]
通讯作者: Cross,CE
Therapeutic Targeting of Myeloperoxidase in Acute Inflammation
Therapeutic Targeting of Myeloperoxidase in Acute Inflammation
Vitamin E metabolism and lung toxicology
Vitamin E metabolism and lung toxicology
海外基金