COMPLEMENT REGULATION BY APOLIPOPROTEIN J
COMPLEMENT REGULATION BY APOLIPOPROTEIN J
批准号:
6107008
负责人:
TUAJUANDA C. JORDAN
金额:
$19.39万
依托单位国家:
美国
项目类别:
财政年份:
1999
资助国家:
美国
项目状态:
已结题
起止时间:
1999-04-01 至 2000-03-31
关键词:
SDS polyacrylamide gel electrophoresis animal tissue apolipoproteins autoradiography biological signal transduction chemical binding complement inhibitors complement pathway cytolysis gel filtration chromatography hemolysis high performance liquid chromatography human tissue ion exchange chromatography molecular site peptide chemical synthesis point mutation polymerization protein purification protein structure function radiation detector radionuclides site directed mutagenesis
中文摘要
长期目标是开发不同的方法来监管
末端补体复合体(TCC)以抑制其细胞溶解
活动而不干扰需要亚分辨率的活动
将该复合体插入膜中,例如在信号转导中。
这将对动脉粥样硬化和阿尔茨海默氏症等领域有所帮助
疾病,两者都涉及补体介导的细胞溶解。特定目标
L将确定载脂蛋白J(ApoJ)与TCC的相互作用
对体外联合和抑制TCC活性具有重要意义。至
针对这个问题,将进行实验,以确定L)
载脂蛋白J和/或其两个亚基中的任何一个是否干扰
将任何单个组件组装到终端综合体中,以及2)
确定apoJ和/或其两个亚基中的任何一个是否可以直接
抑制C9聚合。具体目标2是鉴定apoJ序列
对其与TCC的相互作用和对其的抑制负责。
实验旨在确定功能域,特别是
那些在阿尔法亚基中,使用多肽,并介绍点
通过定点突变识别这些结构域的突变
功能性氨基酸残基。
英文摘要
The long term objective is to develop ways to differentially regulate the
terminal complement complex (TCC) in order to inhibit its cytolytic
activity without interfering with activities that require sublytic
insertion of the complex into membranes, e.g., in signal transduction.
This would be helpful in such areas as atherosclerosis and Alzheimer's
disease, both of which involve complement-mediated cytolysis. Specific aim
l is to determine the apolipoprotein J (apoJ) - TCC interactions that are
important for the association and inhibition of TCC activity in vitro. To
address this question, experiments will be performed to l) ascertain
whether apoJ and/or either of its two subunits interferes with the
assembly of any individual component into the terminal complex, and 2)
determine whether apoJ and/or either of its two subunits can directly
inhibit C9 polymerization. Specific aim 2 is to identify apoJ sequences
responsible for its interaction with and inhibition of the TCC.
Experiments are designed to identify functional domains, specifically
those in the alpha subunit, using peptides, and to introduce point
mutations into these domains via site-directed mutagenesis to identify
functional amino acid residues.
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会议论文
COMPLEMENT REGULATION BY CLUSTERIN
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批准号:6581853
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项目类别:
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资助金额:$14.86万
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财政年份:2002
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负责人:TUAJUANDA C. JORDAN
-
依托单位:
COMPLEMENT REGULATION BY CLUSTERIN
-
批准号:6478788
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项目类别:
-
资助金额:$14.86万
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财政年份:2001
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负责人:TUAJUANDA C. JORDAN
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依托单位:
COMPLEMENT REGULATION BY CLUSTERIN
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批准号:6450662
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项目类别:
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资助金额:$3.85万
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财政年份:2001
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负责人:TUAJUANDA C. JORDAN
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依托单位:
COMPLEMENT REGULATION BY CLUSTERIN
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批准号:6301645
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项目类别:
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资助金额:$3.85万
-
财政年份:2000
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负责人:TUAJUANDA C. JORDAN
-
依托单位:
COMPLEMENT REGULATION BY APOLIPOPROTEIN J
-
批准号:6217782
-
项目类别:
-
资助金额:$19.39万
-
财政年份:1999
-
负责人:TUAJUANDA C. JORDAN
-
依托单位:
COMPLEMENT REGULATION BY APOLIPOPROTEIN J
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项目类别:
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资助金额:$18.75万
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依托单位:
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依托单位:
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批准号:3024559
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项目类别:
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资助金额:$1.54万
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财政年份:1988
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负责人:TUAJUANDA C. JORDAN
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依托单位:
CATABOLISM OF MEVALONATE IN PSEUDOMONAS
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批准号:3024558
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项目类别:
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财政年份:1987
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依托单位:
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财政年份:1986
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依托单位:
海外基金