BIOSPECIFIC FORCE-SENSING INSTRUMENTS

生物特异性力传感仪器

• 批准号: 6015312
• 负责人: MELVIN L GRIEM
• 金额: $ 46.2万
• 依托单位: NANO TECH CORPORATION
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6015312
• 关键词: atomic force microscopy; bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomedical equipment development; chemical bond; ligands; nanotechnology; receptor binding; surface property

The Aim is to further develop and commercialize a new instrument that uniquely senses small specific intermolecular interactions. The Bioprobe Force Microscope (BFM) utilizes functionalized biospecific tips and modified atomic force microscopy controller functions. Phase I found we could measure binding forces between beta-adrenoreceptors (BAR), a biomedically important receptor, and an antagonist drug. The Specific Aims of Phase II are to measure in control and test periods specific binding forces between: (1) membrane proteins, specifically receptors and channels and their ligands, and (2) nonmembrane globular proteins and interacting molecules. Areas have been chosen because of their scientific and clinical potential. Area Resource Persons are experts in these Areas of membrane and globular proteins. They have identified the potential usefulness of the BFM in their area of science and have contributed to the protocols. Phase II focuses on, but is not limited to: (1) membrane proteins including BARs, insulin receptors, oxytocin receptors and sodium channels, and (2) globular proteins including lipoproteins, prions, and proteins that interact with crystals to prevent kidney stones. The principle behind the single product of Phase I has been extended to biomedically important proteins which, in turn, should develop into commercial products and services. The advancements in Phase II will be generalizable to a universe of applications in which it is important to have information on the interaction between molecules. Since Phase I, patent protection has been obtained for the functionalized tips, making the products more attractive commercially. PROPOSED COMMERCIAL APPLICATION The distinctive advantages of AFM technology in the biological force measurement are its extended force range, the ability to make scanning tips biospecific, high spatial resolution, and nanometer control of x, y and z movement. The ability of the biospecific tips, called bioprobes, is to measure the interaction.

其目的是进一步开发和商业化的一种新的仪器，独特的传感小特定的分子间相互作用。生物探针力显微镜（BFM）利用功能化的生物特异性尖端和改进的原子力显微镜控制器功能。 第一阶段发现，我们可以测量β-肾上腺素受体（BAR），一种生物医学上重要的受体，和拮抗剂药物之间的结合力。 第II阶段的具体目的是在对照和试验期间测量：（1）膜蛋白，特别是受体和通道及其配体，和（2）非膜球状蛋白和相互作用分子之间的特异性结合力。 选择这些领域是因为它们具有科学和临床潜力。 区域资源人员是膜和球状蛋白领域的专家。他们已经确定了BFM在其科学领域的潜在用途，并为议定书做出了贡献。 第二阶段的重点是，但不限于：（1）膜蛋白，包括BAR，胰岛素受体，催产素受体和钠通道，和（2）球状蛋白，包括脂蛋白，朊病毒，和蛋白质与晶体相互作用，以防止肾结石。 第一阶段单一产品背后的原则已经扩展到生物医学上重要的蛋白质，反过来，应该发展成商业产品和服务。 第二阶段的进展将推广到一个应用领域，其中重要的是要有关于分子之间相互作用的信息。 自第一阶段以来，功能化尖端已获得专利保护，使产品更具商业吸引力。建议的商业应用AFM技术在生物力测量中的显着优势是其扩展的力范围，使扫描尖端生物特异性，高空间分辨率和纳米控制的x，y和z运动的能力。 生物特异性尖端（称为生物探针）的能力是测量相互作用。