BIOSPECIFIC FORCE-SENSING INSTRUMENTS

目的是进一步开发和商业化一种新的仪器，独特地感知小的特定分子间相互作用。生物探针力显微镜（BFM）利用功能化的生物特异性尖端和改进的原子力显微镜控制器功能。I期研究发现，我们可以测量生物医学上重要的受体-肾上腺素受体（BAR）和拮抗剂之间的结合力。第二阶段的具体目标是在控制和测试期间测量膜蛋白，特别是受体和通道及其配体之间的特定结合力，以及(2)非膜球状蛋白和相互作用分子。选择这些地区是因为它们的科学和临床潜力。区域资源人员是膜蛋白和球状蛋白领域的专家。他们已经确定了BFM在其科学领域的潜在用途，并为议定书作出了贡献。II期研究重点是但不限于：(1)膜蛋白，包括bar、胰岛素受体、催产素受体和钠通道；(2)球状蛋白，包括脂蛋白、朊病毒和与晶体相互作用以预防肾结石的蛋白质。第一阶段的单一产品背后的原则已经扩展到生物医学上重要的蛋白质，反过来，应该发展成商业产品和服务。在第二阶段的进展将推广到一个宇宙的应用，其中重要的是有信息的分子之间的相互作用。从第一阶段开始，功能化的针尖获得了专利保护，使产品在商业上更具吸引力。AFM技术在生物力测量方面的独特优势在于其扩展的力范围，使扫描尖端具有生物特异性的能力，高空间分辨率，以及对x， y和z运动的纳米控制。被称为生物探针的生物特异性尖端的能力是测量相互作用。

The Aim is to further develop and commercialize a new instrument that uniquely senses small specific intermolecular interactions. The Bioprobe Force Microscope (BFM) utilizes functionalized biospecific tips and modified atomic force microscopy controller functions. Phase I found we could measure binding forces between beta-adrenoreceptors (BAR), a biomedically important receptor, and an antagonist drug. The Specific Aims of Phase II are to measure in control and test periods specific binding forces between: (1) membrane proteins, specifically receptors and channels and their ligands, and (2) nonmembrane globular proteins and interacting molecules. Areas have been chosen because of their scientific and clinical potential. Area Resource Persons are experts in these Areas of membrane and globular proteins. They have identified the potential usefulness of the BFM in their area of science and have contributed to the protocols. Phase II focuses on, but is not limited to: (1) membrane proteins including BARs, insulin receptors, oxytocin receptors and sodium channels, and (2) globular proteins including lipoproteins, prions, and proteins that interact with crystals to prevent kidney stones. The principle behind the single product of Phase I has been extended to biomedically important proteins which, in turn, should develop into commercial products and services. The advancements in Phase II will be generalizable to a universe of applications in which it is important to have information on the interaction between molecules. Since Phase I, patent protection has been obtained for the functionalized tips, making the products more attractive commercially. PROPOSED COMMERCIAL APPLICATION The distinctive advantages of AFM technology in the biological force measurement are its extended force range, the ability to make scanning tips biospecific, high spatial resolution, and nanometer control of x, y and z movement. The ability of the biospecific tips, called bioprobes, is to measure the interaction.