BIOSPECIFIC FORCE-SENSING INSTRUMENTS

生物特异性力传感仪器

• 批准号: 6180779
• 负责人: MELVIN L GRIEM
• 金额: $ 32.93万
• 依托单位: NANO TECH CORPORATION
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6180779
• 关键词: atomic force microscopy; bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomedical equipment development; chemical bond; ligands; nanotechnology; receptor binding; surface property

The Aim is to further develop and commercialize a new instrument that uniquely senses small specific intermolecular interactions. The Bioprobe Force Microscope (BFM) utilizes functionalized biospecific tips and modified atomic force microscopy controller functions. Phase I found we could measure binding forces between beta-adrenoreceptors (BAR), a biomedically important receptor, and an antagonist drug. The Specific Aims of Phase II are to measure in control and test periods specific binding forces between: (1) membrane proteins, specifically receptors and channels and their ligands, and (2) nonmembrane globular proteins and interacting molecules. Areas have been chosen because of their scientific and clinical potential. Area Resource Persons are experts in these Areas of membrane and globular proteins. They have identified the potential usefulness of the BFM in their area of science and have contributed to the protocols. Phase II focuses on, but is not limited to: (1) membrane proteins including BARs, insulin receptors, oxytocin receptors and sodium channels, and (2) globular proteins including lipoproteins, prions, and proteins that interact with crystals to prevent kidney stones. The principle behind the single product of Phase I has been extended to biomedically important proteins which, in turn, should develop into commercial products and services. The advancements in Phase II will be generalizable to a universe of applications in which it is important to have information on the interaction between molecules. Since Phase I, patent protection has been obtained for the functionalized tips, making the products more attractive commercially. PROPOSED COMMERCIAL APPLICATION The distinctive advantages of AFM technology in the biological force measurement are its extended force range, the ability to make scanning tips biospecific, high spatial resolution, and nanometer control of x, y and z movement. The ability of the biospecific tips, called bioprobes, is to measure the interaction.

其目的是进一步开发一种新的仪器，并将其商业化，该仪器独特地检测微小的特定分子间相互作用。生物探针力显微镜(BFM)利用功能化的生物专门化提示和改进的原子力显微镜控制器功能。第一阶段我们发现我们可以测量β-肾上腺素受体(BAR)和拮抗剂药物之间的结合力。BAR是一种具有重要生物医学意义的受体。第二阶段的具体目标是在控制和测试期间测量：(1)膜蛋白，特别是受体和通道及其配体之间的特定结合力，以及(2)非膜球状蛋白和相互作用分子之间的特定结合力。之所以选择这些领域，是因为它们具有科学和临床潜力。区域资源专家是膜蛋白和球蛋白领域的专家。他们确定了BFM在其科学领域的潜在用处，并为议定书作出了贡献。第二阶段集中于但不限于：(1)膜蛋白，包括BAR、胰岛素受体、催产素受体和钠通道，以及(2)球状蛋白，包括脂蛋白、普里恩和与晶体相互作用以防止肾结石的蛋白。第一阶段单一产品背后的原则已经扩展到生物医学上的重要蛋白质，这些蛋白质反过来应该发展成商业产品和服务。第二阶段的进展将可推广到各种应用中，在这些应用中，掌握分子之间相互作用的信息是很重要的。从第一阶段开始，功能化的尖端获得了专利保护，使产品在商业上更具吸引力。提议的商业应用AFM技术在生物力测量中的独特优势是它的作用力范围扩大，能够使扫描尖端具有生物专一性，高空间分辨率，以及x、y和z运动的纳米控制。被称为生物探针的生物特异性尖端的能力是测量相互作用。

项目成果

The mechanism of oxidation-induced low-density lipoprotein aggregation: an analogy to colloidal aggregation and beyond?

氧化诱导的低密度脂蛋白聚集的机制：与胶体聚集的类比及其他？

• DOI: 10.1016/s0006-3495(01)75887-0
• 发表时间: 2001
• 期刊: Biophysical journal
• 影响因子: 3.4
• 作者: Xu,S;Lin,B
• 通讯作者: Lin,B

The assembly of amyloidogenic yeast sup35 as assessed by scanning (atomic) force microscopy: an analogy to linear colloidal aggregation?

• DOI: 10.1016/s0006-3495(01)75712-8
• 发表时间: 2001-07
• 期刊: Biophysical journal
• 影响因子: 3.4
• 作者: Shaohua Xu;Brooke Bevis;M. Arnsdorf

Chemical colloids versus biological colloids: a comparative study for the elucidation of the mechanism of protein fiber formation.

化学胶体与生物胶体：阐明蛋白纤维形成机制的比较研究。

• DOI: 10.1021/bi0474719
• 发表时间: 2005
• 期刊: Biochemistry.
• 作者: Xu,Shaohua;Wu,David;Arnsdorf,Morton;Johnson,Robert;Getz,GodfreyS;Cabana,VeneracionG
• 通讯作者: Cabana,VeneracionG