BIOSPECIFIC FORCE SENSING MICROSCOPES

生物特异性力传感显微镜

• 批准号: 2537395
• 负责人: MELVIN L GRIEM
• 金额: $ 6.44万
• 依托单位: NANO TECH CORPORATION
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2537395
• 关键词: aminoalcohol; atomic force microscopy; beta adrenergic receptor; beta antiadrenergic agent; bioengineering /biomedical engineering; biomedical equipment development; endopeptidases; phosphatidylcholines; propranolol; receptor binding; surface property

The Aim is to develop a new instrument that uniquely senses small specific intermolecular interactions. This Bioprobe Force Microscope (BFM) utilizes functionalized biospecific tips and modified atomic force microscopy controller functions. Initial studies strongly suggest we can measure binding forces between adrenoreceptors (BAR), a biomedically important receptor, and an beta-antagonist drug. The Specific Aims of Phase I are to: (1) confirm that we are measuring specific binding forces between receptors and ligands, and (2) functionalize the tip with differing mixtures of receptor/lipid to relate binding forces to receptor density. Phase II will investigate in detail tip characteristics and different receptor tips and perhaps coatings for other purposes; develop software and controller functions; and apply the BFM to real problems in pharmacology and other living science. At the end of the Phase II, we hope to have developed a BFM and various tips which have been demonstrated in a variety of systems to quantitatively study drug/ligand interactions. This would represent the end of the initial stages of basic research. The initial products could then be developed into commercial products as well as into services that ultimately will support a wide variety of investigations. What we have learned should be generalizable to a whole universe of applications in which it is important to have information on the interaction between molecules. PROPOSED COMMERCIAL APPLICATION The BFM should represent a major break- through for investigators in biological, biochemical, biomedical cellular and molecular biologic, medical, pharmacologic including combinatorial chemistry, physiologic, virologic and virtually all living sciences because of its sensitivity in force measurement, high resolution, extremely small sample requirements, and ability to operate in a physiologic environment. The principles will also be useful to the studies of solids, gases and liquids in physics, chemistry, geochemistry and other fields in which nanoscale charge resolution is useful.

目的是开发一种新的仪器，独特的感觉小 特定的分子间相互作用。 这台生物探针力显微镜 (BFM)利用功能化的生物特异性尖端和改进的原子力 显微镜控制器功能。 初步研究强烈表明， 可以测量肾上腺素受体（BAR）之间的结合力， 重要受体和β-拮抗剂药物。 的具体目标 第一阶段是：（1）确认我们正在测量特异性结合 受体和配体之间的力，以及（2）用 受体/脂质的不同混合物，以使结合力与 受体密度 第二阶段将详细调查提示 特性和不同的接收器尖端， 其他目的;开发软件和控制器功能;并应用 BFM应用于药理学和其他生命科学中的真实的问题。 在 在第二阶段结束时，我们希望已经制定了BFM和各种提示 这已经在各种系统中得到了证明， 研究药物/配体相互作用。 这将意味着 基础研究的初始阶段。 最初的产品可以是 发展成商业产品和服务， 最终将支持各种各样的调查。 我们所拥有的 学习应该推广到整个宇宙的应用， 这是很重要的信息之间的相互作用 分子。 拟议的商业应用BFM应代表一个重大突破- 生物学、生物化学、生物医学领域的研究人员 细胞和分子生物学、医学、药理学，包括 组合化学、生理学、病毒学和几乎所有生物学 由于其在力测量中的灵敏度， 分辨率、极小的样品要求和操作能力 在生理环境中。 这些原则也将有助于 物理、化学、地球化学中固体、气体和液体的研究 以及纳米级电荷分辨率有用的其他领域。