OXYGEN OPTRODE FOR CONTINUOUS PROCESSING BIOREACTORS

用于连续处理生物反应器的氧气光极

• 批准号: 2792699
• 负责人: JAMES A KANE
• 金额: $ 37.66万
• 依托单位: POLESTAR TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-30 至 2000-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2792699
• 关键词: analytical chemistry; biomedical equipment development; bioreactors; electrodes; electron optics; fluorescent dye /probe; monitoring device; oxygen; photochemistry

A Small Business Research (SBIR) project is proposed for development of an improved optical oxygen sensor for dissolved oxygen monitoring in the cell culture medium of continuous process bioreactors. The improved oxygen monitor will enable reliable long-term control of oxygen levels in continuous process reactors currently being used in drug and therapeutic protein synthesis. Further, the oxygen monitor will implement an integrated optoelectronic emitter and detector design housed within a one inch header assembly. The output will interface with any PC data acquisition card for numerical oxygen concentration readout and/or process control. The same sensor would also expand the use of many oxygen sensitive cultures not currently implemented because of the inability to accurately monitor medium having low dissolved oxygen concentrations. The specific aim of the Phase I project is the demonstration of the performance of an improved fluorescent quenching based oxygen sensing membrane chemistry in conjunction with fluorescent lifetime detection. The sensor will be evaluated for oxygen response in laboratory calibrations against solutions equilibrated gas mixtures of known composition, examined for effects of autoclaving by repeated calibrations and tested in a functional bioreactor under the low dissolved oxygen condition where current oxygen electrodes fail to provide adequate performance. PROPOSED COMMERCIAL APPLICATION: Success in the applications of modern biotechnological techniques is evidenced by the variety of products, ranging from specialty chemicals, polymers and food, to therapeutic drugs produced by using recombinant DNA methodologies in various cell culture systems. There is constant need to develop and implement a new sensing system to effectively control and optimize these biological processes.

提出了一个小型企业研究(SBIR)项目，用于开发一个 用于细胞内溶解氧监测的改进型光学氧传感器 连续式生物反应器的培养基料。改进的氧气 监控器将能够可靠地长期控制 目前用于药物和治疗的连续过程反应器 蛋白质合成。此外，氧气监测仪将实施 集成光电发射器和探测器设计，集于一体 英制集箱组件。输出将与任何PC数据接口 用于数字氧气浓度读数和/或处理的采集卡 控制力。同样的传感器也将扩大许多氧气的使用 敏感的区域性当前未实施，因为无法 准确监测溶氧浓度较低的介质。这个 一期工程的具体目标是示范 一种改进的基于荧光猝灭的氧传感器的性能 膜化学与荧光寿命检测相结合。 该传感器将在实验室中进行氧气响应评估。 已知的平衡混合气体溶液的校准 通过重复校准检查高压灭菌效果的组合物 并在功能生物反应器中进行了低溶解氧条件下的测试 当前氧电极不能提供足够的氧气的状态 性能。 拟议的商业应用：现代应用的成功 生物技术技术从产品的多样性中得到了证明， 从特种化学品、聚合物和食品到治疗药物 在各种细胞培养中使用重组DNA方法生产的 系统。不断地需要开发和实现一种新的传感 系统，以有效地控制和优化这些生物过程。