GLUCOSE INDICATOR FOR IN SITU BIOREACTOR MONITORING

用于原位生物反应器监测的葡萄糖指示剂

• 批准号: 2424324
• 负责人: JAMES A KANE
• 金额: $ 9.98万
• 依托单位: POLESTAR TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-30 至 1998-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2424324
• 关键词: bioreactors; boronic acids; chemical binding; chemical synthesis; concanavalin A; electron optics; fluorescent dye /probe; fluorimetry; glucose; method development; monitoring device; polymers; reagent /indicator

The goal of this Small Business Innovation Research project is to demonstrate a fluorescent glucose-indicator chemistry with high thermal stability for construction of an in situ optical glucose sensor to monitor the culture medium of continuous process bioreactors. The specific binding properties. The new polymer will be evaluated for use in a competitive binding glucose assay using fluorescence resonance energy transfer quenching to detect glucose levels in the culture medium. The evaluation will include characterization of the glucose binding polymer for molecular weight and composition and spectrofluorimetric titration of the proposed indicator chemistry with glucose and known culture medium analytes and metabolites. Spectral data will be examined for glucose sensitivity and selectivity over significant reactor concentration ranges. An in situ glucose probe would improve the ability to control the physiological state of glucose substrate reactors while reducing the cost and complexity. The probe would also provide the type of real time glucose concentrations necessary for substrate limiting culture operation. Improvements in bioreactor performance will aid the ongoing efforts to develop new drugs and therapeutic proteins using biotechnology. PROPOSED COMMERCIAL APPLICATION: Success in the applications of modern biotechnological techniques is evidenced by the variety of products, ranging from specialty chemicals, polymers and food, to therapeutic drugs produced by using recombinant DNA methodologies in various cell culture systems. There is constant need to develop and implement a new sensing system to effectively control and optimize these biological processes.

这个小企业创新研究项目的目标是 展示具有高热的荧光葡萄糖指示剂化学 构建原位光学葡萄糖传感器进行监测的稳定性 连续过程生物反应器的培养基。 具体的 绑定属性。 新聚合物将被评估用于 使用荧光共振能量的竞争性结合葡萄糖测定 转移淬灭以检测培养基中的葡萄糖水平。 这 评估将包括葡萄糖结合聚合物的表征 用于分子量和组成以及荧光分光滴定 所提议的指示剂与葡萄糖和已知培养基的化学反应 分析物和代谢物。 将检查葡萄糖的光谱数据 在重要的反应器浓度范围内的灵敏度和选择性。 原位葡萄糖探针将提高控制 葡萄糖底物反应器的生理状态，同时降低成本 和复杂性。 该探测器还将提供实时类型 底物限制培养操作所需的葡萄糖浓度。 生物反应器性能的改进将有助于持续努力 利用生物技术开发新药和治疗性蛋白质。 拟议的商业应用：现代技术应用的成功 生物技术通过产品的多样性得到证明， 范围从特种化学品、聚合物和食品到治疗药物 在各种细胞培养物中使用重组 DNA 方法生产 系统。 不断需要开发和实施新的传感技术 系统有效地控制和优化这些生物过程。