OXYGEN OPTRODE FOR CONTINUOUS PROCESSING BIOREACTORS

用于连续处理生物反应器的氧气光极

• 批准号: 6139587
• 负责人: JAMES A KANE
• 金额: $ 35.85万
• 依托单位: POLESTAR TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-30 至 2000-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6139587
• 关键词: analytical chemistry; biomedical equipment development; bioreactors; electrodes; electron optics; fluorescent dye /probe; monitoring device; oxygen; photochemistry

A Small Business Research (SBIR) project is proposed for development of an improved optical oxygen sensor for dissolved oxygen monitoring in the cell culture medium of continuous process bioreactors. The improved oxygen monitor will enable reliable long-term control of oxygen levels in continuous process reactors currently being used in drug and therapeutic protein synthesis. Further, the oxygen monitor will implement an integrated optoelectronic emitter and detector design housed within a one inch header assembly. The output will interface with any PC data acquisition card for numerical oxygen concentration readout and/or process control. The same sensor would also expand the use of many oxygen sensitive cultures not currently implemented because of the inability to accurately monitor medium having low dissolved oxygen concentrations. The specific aim of the Phase I project is the demonstration of the performance of an improved fluorescent quenching based oxygen sensing membrane chemistry in conjunction with fluorescent lifetime detection. The sensor will be evaluated for oxygen response in laboratory calibrations against solutions equilibrated gas mixtures of known composition, examined for effects of autoclaving by repeated calibrations and tested in a functional bioreactor under the low dissolved oxygen condition where current oxygen electrodes fail to provide adequate performance. PROPOSED COMMERCIAL APPLICATION: Success in the applications of modern biotechnological techniques is evidenced by the variety of products, ranging from specialty chemicals, polymers and food, to therapeutic drugs produced by using recombinant DNA methodologies in various cell culture systems. There is constant need to develop and implement a new sensing system to effectively control and optimize these biological processes.

一个小企业研究（SBIR）项目被提议用于开发一个 用于电解槽中溶解氧监测的改进的光学氧传感器 连续工艺生物反应器的培养基。 改善的氧气 监测器将能够可靠地长期控制氧气水平， 目前用于药物和治疗的连续过程反应器 蛋白质合成 此外，氧气监测器将实施 集成光电发射器和检测器设计， 英寸集管组件。 输出将与任何PC数据接口 用于氧浓度数值读出和/或处理的采集卡 控制 同样的传感器也将扩大许多氧气的使用 敏感文化目前没有实施，因为无法 准确监测具有低溶解氧浓度的介质。 的 第一阶段项目的具体目标是展示 改进的基于荧光猝灭的氧传感器的性能 膜化学结合荧光寿命检测。 传感器将在实验室进行氧响应评估 对已知的平衡气体混合物的溶液进行校准 组合物，通过重复校准检查高压灭菌的影响 并在功能性生物反应器中在低溶解氧下进行测试 当前氧电极不能提供足够的 性能 建议的商业应用：在现代化的应用中取得成功 生物技术通过产品的多样性来证明， 从特种化学品、聚合物和食品到治疗药物， 通过使用重组DNA方法在各种细胞培养物中产生 系统. 不断需要开发和实施一种新的传感技术， 系统，以有效地控制和优化这些生物过程。