TRANSCRIPTIONAL CONTROL OF OSTEOBLAST STRESS RESPONSES

描述：本职业发展计划的目标是促进 成骨细胞生物学和骨内植入物相关活动， 基因转录的基础研究，建立一个实验室， 将为学生、研究员和 临床科学家，并发展临床研究兴趣的支持 并对这些研究兴趣进行补充。 完成这项工作的方法是 a）一年的正规培训， 分子生物学实验室，B）四年75%的研究 为实现研究的具体目标而付出的努力 项目 目的是调查：1）转录抑制作为一种 热休克蛋白27的组织特异性组成型表达机制; 2） 成骨细胞中hsp 27转录的雌激素控制;和3） hsp 27在成骨细胞中的伴侣蛋白功能。 罗兰阿诺德博士， 牙科研究中心，将作为这一职业的主要赞助商 发展计划。 库珀博士计划参加两个相关的基础研究 会议/专题讨论会，而其他前往牙科研究会议 将由申请人的R29奖和部门基金支付。 卡罗莱纳转基因小鼠讲习班， 肽噬菌体展示库，并在生物医学显微镜计划 并为奖励期的前三年编制预算。

DESCRIPTION: The goals of this career development plan are to contribute to osteoblast biology and endosseous implant related activities through fundamental studies of gene transcription, to establish a laboratory that will provide exemplary training of students, fellows, and clinician-scientists, and to develop clinical research interests supported by and complimentary to these research interests. This will be achieved through a) one year of formal training in a combination of three leading molecular biology laboratories at UNC, and b) four years of 75% research effort devoted towards accomplishing the specific aims of the research project. The aims are to investigate: 1) transcriptional suppression as a mechanism for tissue-specific constitutive expression of hsp27; 2) estrogenic control of hsp27 transcription in osteoblasts; and 3) the chaperone function of hsp27 in osteoblasts. Dr. Roland Arnold, Director of the Dental Research Center, will serve as lead sponsor of this career development program. Dr. Cooper plans to attend two relevant basic research meetings/symposia per year, while other travel to dental research meetings will be covered by the applicant s R29 award and departmental funds. Carolina Workshops on transgenic mice, on construction and screening of peptide phage display libraries, and on biomedical microscopy are planned and budgeted for the first three years of the award period.