TRANSCRIPTIONAL CONTROL OF OSTEOBLAST STRESS RESPONSES

成骨细胞应激反应的转录控制

• 批准号: 6176685
• 负责人: LYNDON F COOPER
• 金额: $ 7.86万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-01 至 2001-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6176685
• 关键词: actins; endosseous dental implant; estrogen receptors; gene induction /repression; genetic transcription; genetically modified animals; laboratory mouse; medical implant science; medical rehabilitation related tag; osteoblasts; recombinant proteins; stress proteins

DESCRIPTION: The goals of this career development plan are to contribute to osteoblast biology and endosseous implant related activities through fundamental studies of gene transcription, to establish a laboratory that will provide exemplary training of students, fellows, and clinician-scientists, and to develop clinical research interests supported by and complimentary to these research interests. This will be achieved through a) one year of formal training in a combination of three leading molecular biology laboratories at UNC, and b) four years of 75% research effort devoted towards accomplishing the specific aims of the research project. The aims are to investigate: 1) transcriptional suppression as a mechanism for tissue-specific constitutive expression of hsp27; 2) estrogenic control of hsp27 transcription in osteoblasts; and 3) the chaperone function of hsp27 in osteoblasts. Dr. Roland Arnold, Director of the Dental Research Center, will serve as lead sponsor of this career development program. Dr. Cooper plans to attend two relevant basic research meetings/symposia per year, while other travel to dental research meetings will be covered by the applicant s R29 award and departmental funds. Carolina Workshops on transgenic mice, on construction and screening of peptide phage display libraries, and on biomedical microscopy are planned and budgeted for the first three years of the award period.

描述：这项职业发展计划的目标是为 成骨细胞生物学与骨内种植相关活动 基因转录的基础研究，建立一个实验室， 将为学生、研究员和 临床医生-科学家，并支持开发临床研究兴趣 是对这些研究兴趣的补充和补充。这将会实现的 通过a)一年的正式培训，在三个领导组合 北卡罗来纳大学的分子生物学实验室，以及b)四年75%的研究 致力于实现研究的具体目标的努力 项目。其目的是调查：1)转录抑制作为一种 Hsp27的组织特异性结构性表达机制；2) 成骨细胞中HSP27转录的雌激素调控；3) 热休克蛋白27在成骨细胞中的伴侣功能罗兰·阿诺德博士，总干事 牙科研究中心将作为这项事业的主要赞助商 发展计划。库珀博士计划参加两项相关的基础研究 每年举行会议/座谈会，而其他人则出差参加牙科研究会议 将由申请者S R29奖励和部门资金支付。 卡罗莱纳转基因小鼠研讨会，关于转基因小鼠的构建和筛选 多肽噬菌体展示文库和生物医学显微镜计划 并为获奖期的前三年编入预算。