CORE--MOLECULAR BIOLOGY

分子生物学技术已经彻底改变了 生殖生物学家将在一项 快速、灵敏的态度。然而，这种方法通常需要大量的 投资时间以获得适当的专业知识和设备， 在一个快速发展的领域中保持最新的时间承诺，以及 对质量控制的严格关注，即使是最常规和 重复的技术。许多分子实验的一个主要成本是 提供可靠的试剂和探头所需的人力。一个集中的、 配备完善的核心是最高效、最经济的方式 项目追求创新的重组DNA方法学和试剂 生殖医学中的分子工作。分子科学的目标 核心是为研究项目提供服务、专业知识和设备 解决生殖方面的临床和基本问题。三项研究 U54内的项目，以及由U54批准的另外五个项目 生殖科学科需要进入核心进行例行检查 探针标记服务(末端标记、随机标记和单链标记 RNA核探针)、DNA分离、质粒生长和纯化、DNA 测序，以及DNA片段分离和亚克隆程序。其核心是 总监和人员将提供有关设计的建议和专业知识 探针和表达载体，各种RNA和DNA定量方法， 以及图书馆筛选等专门技术。核心人员将 提供及时、有质量控制的服务和试剂 在优先基础上开展项目。批准调查人员可以访问核心 对于专门的设备，以在 可获得性基础。最后，核心人员将继续改进和 根据项目需要开发其他服务和程序 访问核心，并更新当前服务以提供最多 灵敏、准确、经济实惠的试剂。

Molecular biology techniques have revolutionized the ability of reproductive biologists to address specific physiological questions in a rapid, sensitive manner. This approach, however, often requires a large time investment to acquire appropriate expertise and equipment, additional time commitments to keep current in a rapidly advancing field, and critical attention to quality control for even the most routine and repetitive techniques. A major cost for many molecular experiments is the labor involved to provide dependable reagents and probes. A centralized, well-equipped core is the most efficient and economical way to provide recombinant DNA methodology and reagents for projects to pursue innovate molecular work in reproductive medicine. The objective of the Molecular Core is to provide services, expertise, and equipment to research projects addressing clinical and basic questions in reproduction. Three research projects within the U54, and five additional projects approved by the Reproductive Science Branch require access to the Core for routine services of probe labeling (end-label, random priming, and single-strand RNA riboprobes), DNA isolation, plasmid growths and purification, DNA sequencing, and DNA fragment isolation and subcloning procedures. The core director and personnel will provide advice and expertise on the design of probes and expression vectors, various RNA and DNA quantitation methods, and specialized techniques such as library screening. Core personnel will provide timely, quality-controlled service and reagents to approved projects on a priority basis. Approve investigators can access the core for specialized equipment to perform more advanced techniques on a availability basis. Finally, Core personnel will continue to improve and develop additional services and procedures as required by projects accessing the core, and update current services to provide the most sensitive, precise, and cost-effective reagents.