CORE--MOLECULAR BIOLOGY FACILITY

分子生物学技术已经彻底改变了生殖生物学家以快速、敏感的方式解决特定生理问题的能力。然而，这种方法往往需要大量的时间投资来获得适当的专业知识和设备，额外的时间承诺来保持在一个快速发展的领域中的最新情况，并对即使是最常规和最重复的技术的质量控制也给予严格的关注。许多分子实验的主要成本是提供可靠的试剂和探针所涉及的劳动力。一个集中的、装备精良的核心是提供重组DNA最有效和最经济的方式 生殖医学创新分子研究项目的方法学和试剂。分子核心的目标是为解决生殖临床和基本问题的研究项目提供服务、专业知识和设备。U54内的三个研究项目和生殖科学处批准的另外五个项目需要进入核心，以提供探针标记(末端标记、随机引爆和单链RNA核探针)、DNA分离、质粒生长和纯化等常规服务， DNA测序，以及DNA片段分离和亚克隆程序。核心主任和人员将在探针和表达载体的设计、各种RNA和DNA定量方法以及库筛选等专门技术方面提供建议和专业知识。核心人员将优先为批准的项目提供及时、有质量控制的服务和试剂。经批准的调查人员可以进入核心 在可获得性的基础上提供专门的设备以执行更先进的技术。最后，核心人员将继续改进和发展访问核心的项目所需的额外服务和程序，并更新现有服务，以提供最敏感、最准确和最具成本效益的试剂。

Molecular biology techniques have revolutionized the ability of reproductive biologists to address specific physiological questions in a rapid, sensitive manner. This approach, however, often requires a large time investment to acquire appropriate expertise and equipment, additional time commitments to keep current in a rapidly advancing field, and critical attention to quality control for even the most routine and repetitive techniques. A major cost for many molecular experiments is the labor involved to provide dependable reagents and probes. A centralized, well-equipped core is the most efficient and economical way to provide recombinant DNA methodology and reagents for projects to pursue innovative molecular work in reproductive medicine. The objective of the Molecular Core is to provide services, expertise, and equipment to research projects addressing clinical and basic questions in reproduction. Three research projects within the U54, and five additional projects approved by the Reproductive Sciences Branch require access to the Core for routine services of probe labeling (end-label, random priming, and single-strand RNA riboprobes), DNA isolation, plasmid growths and purification, DNA sequencing, and DNA fragment isolation and subcloning procedures. The core director and personnel will provide advice and expertise on the design of probes and expression vectors, various RNA and DNA quantitation methods, and specialized techniques such as library screening. Core personnel will provide timely, quality controlled service and reagents to approved projects on a priority basis. Approved investigators can access the core for specialized equipment to perform more advanced techniques on an availability basis. Finally, Core personnel will continue to improve and develop additional services and procedures as required by projects accessing the core, and update current services to provide the most sensitive, precise, and cost-effective reagents.