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Molecular Electron Microscopy Core Facility Improvements

分子电子显微镜核心设施的改进

基本信息

批准号：
7937627
负责人：
MARGARET A SHUPNIK
金额：
$ 204.06万
依托单位：
UNIVERSITY OF VIRGINIA
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-03-25 至 2013-06-23
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7937627
关键词：
10 year old Arts Biochemistry Biological Biological Sciences Buffers Collaborations Communication Communities Computers Core Facility Cryoelectron Microscopy Development Electromagnetics Electron Microscope Electron Microscopy Electron Microscopy Facility Exhibits Faculty Film Fostering Freezing Funding Height Housekeeping Image Jordan Laboratories Location Maintenance Manufacturer Name Microscope Molecular Molecular Genetics Occupations Performance Physics Physiology Positioning Attribute Preparation Productivity Research Research Personnel Resolution Resources Robotics Sampling Schools Scientist Site Specimen Structure Students Titan Translational Research Universities Virginia Visit Weight-Bearing state Work aqueous design design and construction macromolecule medical schools meetings member operation professor square foot structural biology vibration

项目摘要

DESCRIPTION (provided by applicant): This G20 proposal from the University of Virginia School of Medicine (UVA SOM) requests $2.1 M to upgrade and consolidate the UVA SOM electron microscopy (EM) resources into a Molecular Electron Microscopy Core (MEMC) facility. We estimate that the associated design, construction, and maintenance work will create or maintain ~45 jobs for the community, and create and/or maintain up to 4 positions for researchers, computer programmers, and housekeeping staff. There are currently four electron microscopes in use within the SOM for structural biology. All of these are at least ten years old, and one has been declared obsolete by the manufacturer and can no longer be properly maintained. Two modern, high-performance electron microscopes have already been purchased -- a robotic FEI Titan Krios and an FEI F20. These electron microscopes are designed for recording high-resolution images of biological macromolecules by electron cryomicroscopy (cryoEM), in which the specimen is maintained in frozen, vitrified aqueous buffer, thereby preserving native biological structure. The current locations for the EM facilities in Old Jordan Hall and the Snyder Building not only lack the requisite ceiling height and load-bearing capacity, but also exhibit too much vibration and electromagnetic interference for the new microscopes to operate within specifications. The proposal will replace the scattered EM laboratories with one central MEMC laboratory located in the Life Sciences Annex adjacent to the Snyder Translational Science Building at the Fontaine Research Campus. The 2,100 sq ft MEMC facility will contain two environmentally regulated, electromagnetically shielded, and acoustically isolated microscope rooms. In addition, there will be a control room for remote operation of the microscopes. Adjoining laboratory space will allow on-site sample preparation, and a darkroom will be available for film development. The new location is necessary to accommodate the height of the new state-of-the-art FEI Titan Krios microscope and to meet the stringent requirements for optimal performance. The MEMC facility will be co-directed by Dr. Mark Yeager, Professor and Chair of the Department of Molecular Physiology and Biological Physics and Dr. Edward Egelman, Professor of Biochemistry and Molecular Genetics. The improvements provided by the MEMC facility will (1) enhance the quality of research at UVA, (2) increase scientific productivity, (3) foster communication, interactions and collaboration amongst scientists, (4) boost the ability of UVA to attract and maintain premier faculty and students, and (5) strengthen the competitiveness of our faculty for funding. The MEMC facility will not only be available to SOM faculty members but also to other scientists in other schools and departments at UVA. This will be a unique Core laboratory at UVA, and we envision that scientists throughout Virginia and the surrounding states will visit UVA to take advantage of such a cutting-edge laboratory for performing molecular resolution electron microscopy.

描述（由申请人提供）：弗吉尼亚大学医学院（UVA SOM）的这份G20提案要求210万美元将UVA SOM电子显微镜（EM）资源升级和整合为分子电子显微镜核心（MEMC）设施。我们估计，相关的设计、施工和维护工作将为社区创造或维持约45个工作岗位，并为研究人员、计算机程序员和家政人员创造和/或维持多达4个职位。目前，SOM中有四台用于结构生物学的电子显微镜。所有这些都至少有十年的历史，其中一个已经被制造商宣布过时，不能再进行适当的维护。已经购买了两台现代化的高性能电子显微镜-FEI Titan Krios机器人和FEI F20。这些电子显微镜设计用于通过电子低温显微镜（cryoEM）记录生物大分子的高分辨率图像，其中样品保持在冷冻的玻璃化水性缓冲液中，从而保留天然生物结构。目前位于Old Jordan Hall和Snyder Building的EM设施不仅缺乏必要的天花板高度和承重能力，而且对于新显微镜来说，振动和电磁干扰太大，无法在规范范围内运行。该提案将取代分散的EM实验室，一个中央MEMC实验室位于生命科学附件毗邻斯奈德转化科学大楼在方丹研究园区。2，100平方英尺的MEMC设施将包含两个环境监管，电磁屏蔽和声学隔离的显微镜室。此外，将有一个控制室，用于远程操作显微镜。相邻的实验室空间将允许现场样品制备，暗室将用于胶片显影。新的位置是必要的，以适应最先进的FEI Titan Krios显微镜的高度，并满足最佳性能的严格要求。MEMC设施将由分子生理学和生物物理学系教授兼系主任Mark Yeager博士和生物化学和分子遗传学教授Edward Egelman博士共同指导。 MEMC设施提供的改进将（1）提高UVA的研究质量，（2）提高科学生产力，（3）促进科学家之间的沟通，互动和合作，（4）提高UVA吸引和维持一流教师和学生的能力，以及（5）加强我们教师的竞争力。MEMC设施将不仅提供给SOM教职员工，也提供给UVA其他学校和部门的其他科学家。这将是UVA独特的核心实验室，我们设想整个弗吉尼亚州和周边各州的科学家将访问UVA，以利用这样一个尖端的实验室进行分子分辨率电子显微镜。