CORE--MOLECULAR BIOLOGY

核心--分子生物学

• 批准号: 6440529
• 负责人: MARGARET A SHUPNIK
• 金额: $ 17.42万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6440529
• 关键词: biomedical facility; genetic library; messenger RNA; method development; molecular biology; molecular cloning; nucleic acid probes; nucleic acid quantitation /detection; nucleic acid sequence; polymerase chain reaction; recombinant DNA; reproduction; site directed mutagenesis; training; transfection

Molecular biology techniques have revolutionized the ability of reproductive biologists to address specific physiological questions in a rapid, sensitive manner. This approach, however, often requires a large time investment to acquire appropriate expertise and equipment, additional time commitments to keep current in a rapidly advancing field, and critical attention to quality control for even the most routine and repetitive techniques. A major cost for many molecular experiments is the labor involved to provide dependable reagents and probes. A centralized, well-equipped core is the most efficient and economical way to provide recombinant DNA methodology and reagents for projects to pursue innovate molecular work in reproductive medicine. The objective of the Molecular Core is to provide services, expertise, and equipment to research projects addressing clinical and basic questions in reproduction. Three research projects within the U54, and five additional projects approved by the Reproductive Science Branch require access to the Core for routine services of probe labeling (end-label, random priming, and single-strand RNA riboprobes), DNA isolation, plasmid growths and purification, DNA sequencing, and DNA fragment isolation and subcloning procedures. The core director and personnel will provide advice and expertise on the design of probes and expression vectors, various RNA and DNA quantitation methods, and specialized techniques such as library screening. Core personnel will provide timely, quality-controlled service and reagents to approved projects on a priority basis. Approve investigators can access the core for specialized equipment to perform more advanced techniques on a availability basis. Finally, Core personnel will continue to improve and develop additional services and procedures as required by projects accessing the core, and update current services to provide the most sensitive, precise, and cost-effective reagents.

分子生物学技术彻底改变了 生殖生物学家解决特定的生理问题 快速、灵敏的方式。然而，这种方法通常需要大量 时间投资以获得适当的专业知识和设备，额外 投入时间以跟上快速发展的领域的潮流，以及 即使是最常规和最严格的质量控制 重复的技术。许多分子实验的主要成本是 提供可靠的试剂和探针所需的劳动力。一个集中的、 装备精良的核心是提供最高效、最经济的方式 用于追求创新项目的重组 DNA 方法和试剂 生殖医学中的分子工作。分子的目标 核心是为研究项目提供服务、专业知识和设备 解决生殖中的临床和基本问题。三研究 U54 内的项目，以及经 U54 批准的另外五个项目 生殖科学部门需要访问核心进行日常工作 探针标记服务（末端标记、随机引物和单链 RNA 核糖核酸探针）、DNA 分离、质粒生长和纯化、DNA 测序、DNA 片段分离和亚克隆程序。核心 董事和人员将提供有关设计的建议和专业知识 探针和表达载体，各种RNA和DNA定量方法， 以及文库筛选等专业技术。核心人员将 为批准的人员提供及时、质量控制的服务和试剂 项目优先。批准调查员可以访问核心 用于专业设备来执行更先进的技术 可用性基础。最后，核心人员将不断提升和 根据项目要求开发额外的服务和程序 访问核心，并更新当前服务以提供最 灵敏、精确且经济高效的试剂。