DEVELOPMENT OF A WHOLE-BLOOD PLATELET AGGREGOMETER

全血血小板聚集仪的开发

• 批准号: 6071729
• 负责人: SYED F MOHAMMAD
• 金额: $ 9.63万
• 依托单位: THROMBODYNE, INC.
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-15 至 2001-03-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6071729
• 关键词: biomedical equipment development; human tissue; lasers; light scattering; measurement; platelet activation; platelet aggregation; platelet disorder

This a request for investigation and development of a diagnostic tool to assess platelet function; the instrument will utilize a laser light.scattering method to detect platelet aggregates in blood. Current platelet aggregometers assess platelet aggregation either in media free of erythrocytes, or an indirect approach is used to extrapolate information relating to platelet aggregation. The applicants have tested the feasibility of utilizing the light-scattering concept for detection of platelet aggregates in the presence of all blood cells (whole blood). The promise shown by preliminary results has prompted the efforts for development of a whole blood aggregometer. Platelet aggregometers can be found in nearly all reasonably equipped hemostasis laboratories; they are widely used to examine platelet function in the blood of patients with abnormal hemostasis. The applicants anticipate that the light-scattering whole blood aggregometer will greatly facilitate the performance of aggregation tests, it will be more cost-effective, and minimize the risk of operator contact with blood. It will assess platelet function in more physiologically relevant conditions and it has the potential of being more sensitive in its ability to detect platelet abnormalities. The work will entail development of a suitable sample chamber, optimization of signal detection scheme and proper algorithms for data processing and display of results. PROPOSED COMMERCIAL APPLICATIONS: This research will lead to the development of a device which will have applications in clinical laboratories. The device will test the functional status of blood platelets and help in identifying abnormalities in these cells. The device will permit assessment of platelet function in undiluted whole blood; this is not possible with the existing aggregometers.

这是一项调查和开发评估血小板功能的诊断工具的请求；该仪器将利用激光散射方法来检测血液中的血小板聚集体。目前的血小板聚集计要么在不含红细胞的介质中评估血小板聚集，要么使用间接方法来推断与血小板聚集相关的信息。申请人已经测试了利用光散射概念在所有血细胞（全血）存在的情况下检测血小板聚集体的可行性。初步结果所显示的希望促使人们努力开发全血凝集仪。几乎所有装备合理的止血实验室都配备有血小板聚集仪；它们广泛用于检查止血异常患者血液中的血小板功能。申请人预计光散射全血聚集仪将极大地促进聚集测试的进行，其将更具成本效益，并且将操作者接触血液的风险降至最低。它将在更生理相关的条件下评估血小板功能，并且有可能在检测血小板异常的能力方面更加敏感。这项工作将需要开发合适的样品室、优化信号检测方案以及用于数据处理和结果显示的适当算法。拟议的商业应用：这项研究将导致开发一种可应用于临床实验室的设备。该设备将测试血小板的功能状态，并帮助识别这些细胞的异常情况。该装置将允许评估未稀释全血中的血小板功能；这对于现有的聚合计来说是不可能的。