IMPROVED BIOCATALYST FOR EPOXIDE PRODUCTION

描述：（改编自申请人的摘要）本阶段SBIR项目 涉及开发新的生物催化剂，用于生产对映体纯 用于化学合成的环氧化物。环氧化物的反应性使它们 用于许多工业化学合成的有用和重要的中间体， 包括药品、农用化学品和聚合物的生产。 尽管外消旋环氧化物目前有许多应用，但需求 用于生产对映体纯的原料的方法近年来有所增加 因为已知单个对映体可以具有显著的 不同的化学和药物特性。此外，通过使用 对映体纯的构建单元来生产药物， 对映体纯，药物批准过程的成本可以是 因为只需要测试一种化合物的功效， 和安全性 在这个项目中，申请人将修改克隆的单加氧酶基因 （酶）已知产生环氧化物以提高其环氧化速率， 对映体选择性该项目将涉及网站的组合 定向和随机诱变，以及新的筛选和 用于鉴定和选择有用突变体的选择方法。实验 也将进行优化生产环氧化物在生物反应器中 系统. 拟定商业应用： 该项目生产的生物催化剂和对映体纯环氧化物将具有 广泛应用于化工中间体。 这些化合物将是有用的 适用于制药、农业、聚合物和精细化工行业。

DESCRIPTION: (Adapted from the applicant's abstract) This Phase I SBIR project involves the development of new biocatalysts for producing enantio-pure epoxides for use in chemical synthesis. The reactivity of epoxides makes them useful and important intermediates for many industrial chemical syntheses, including the production of pharmaceuticals, agrochemicals, and polymers. Although there are many current applications for racemic epoxides, the demand for the production of enantiomerically pure feedstocks has increased in recent years because it is known that individual enantiomers can have dramatically different chemical and pharmaceutical properties. Furthermore, by using enantio-pure building blocks to produce pharmaceuticals that are enantiomerically pure, the cost of the drug approval process can be significantly reduced because only one compound needs to be tested for efficacy and safety. During this project, the applicants will modify cloned monooxygenase genes (enzymes) known to produce epoxides to improve their epoxidation rate and enantiomeric selectivity. The project will involve a combination of site directed and random mutagenesis, and the development of novel screening and selection processes for identifying and selecting useful mutants. Experiments also will be performed to optimize the production of epoxides in bioreactor systems. PROPOSED COMMERCIAL APPLICATION: The biocatalysts and enantio-pure epoxides produced during this project will have a wide range of applications as chemical intermediates. The compounds will be useful to the pharmaceutical, agricultural, polymer, and fine chemical industries.