NOVEL METHOD TO MEASURE BILE ACID TRANSPORT

这是BioPhysics Assay Laboratories， Inc.的一项新的I期申请，该公司成立于1997年，只有四名员工，提议验证和进一步开发一种测量胆汁酸运输的新方法。该项目将重点关注通过两种不同的螯合物标记不同稳定同位素的胆酸。第一阶段的实验将寻求评估标记效率、总电荷、在温度变化的缓冲液中孵育的稳定性、在竞争螯合物和血清环境中挑战的稳定性、检测的敏感性以及使用分离肝细胞的生物活性保留。总体目标是开发一种易于使用和成本有效的标记胆汁酸测定试剂盒，适用于研究和临床应用。目前还没有常规的化验来执行这个功能。建议的商业应用：不可用

This is a new Phase I application from BioPhysics Assay Laboratories, Inc. a company founded in 1997 with four employees that proposes to validate and further develop a novel method to measure bile acid transport. The project will focus on cholic acid labeled with different stable isotopes via two different chelates. Experiments in this phase I proposal will seek to evalulate labeling efficiency, overall charge, stability to incubation in buffer with temperature variation, stability to challenge with competing chelates and in a serum environment, sensitivity of detection, and retention of biological activity using isolated hepatocytes. The overall goal is to develop an easy to use and cost effective labeled bile acid assay kit, suitable for research and clinical applications. There is no current routine assay to perform this function. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE