NOVEL METHOD TO MEASURE GLOMERULAR SIZE-SELECTIVITY

测量肾小球尺寸选择性的新方法

• 批准号: 6014886
• 负责人: CHRISTOPHER P REINHARDT
• 金额: $ 10万
• 依托单位: BIOPAL, INC
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6014886
• 关键词: glomerular filtration rate; kidney function; method development; molecular size; polymers; radiotracer; renal glomerulus

Our specific aim is to validate and further develop an innovative method to measure glomerular size-selectivity. We propose to use a series of novelly labeled, pre-sized inert polymers. By this method, glomerular size-selectivity measurements can be directly obtained from collected serum and urine samples. This approach will eliminate both the disadvantages associated with radioactivity and the cumbersome step of post-separating macromolecules fragments by size-exclusive chromatography. This approach will provide a glomerular size-selectivity assay kit that is safe, easy-to-use, accurate, and cost-effective while providing a diagnostic modality that is versatile for a wide range of research and clinical situations. Experiments will characterize our novel labeling system. Labeled polymer will be tested for the number of labels bond per polymer; overall change; stability to incubation in buffer with temperature variation; stability to challenge with competing chelates and in a serum environment. The optimum labeling conditions will be defined as well as the sensitivity of detection. PROPOSED COMMERCIAL APPLICATION: Research is directed toward the development of an easy-to-use and cost-effective glomerular size-selectivity assay kit, suitable for both the research and clinical marketplace. This product will provide increased assay design flexibility over current methods.

我们的具体目标是验证和进一步开发一种创新的方法来测量肾小球的大小选择性。我们建议使用一系列新颖的标记，预大小的惰性聚合物。通过这种方法，肾小球大小选择性测量可以直接从收集的血清和尿液样品中获得。这种方法将消除与放射性相关的缺点和通过尺寸排阻色谱法后分离大分子片段的繁琐步骤。这种方法将提供一种安全、易于使用、准确和具有成本效益的肾小球大小选择性测定试剂盒，同时提供一种适用于广泛研究和临床情况的诊断模式。实验将描述我们的新标记系统。将检测标记聚合物中每个聚合物结合的标记物数量;总体变化;在温度变化的缓冲液中孵育的稳定性;在竞争性螯合物和血清环境中挑战的稳定性。将定义最佳标记条件以及检测灵敏度。 拟定商业应用： 研究是针对开发一种易于使用和具有成本效益的肾小球大小选择性测定试剂盒，适用于研究和临床市场。该产品将提供比当前方法更高的检测设计灵活性。