SOFTWARE FOR IMPROVED USE OF THE COLUMN IN HPLC

用于高效液相色谱 (HPLC) 的烷基硅胶柱（例如 C8、C18）制造商目前无法保证不同生产批次的标称等效柱填料能够对不同样品提供相同的保留和分离效果。这反过来又会在 HPLC 方法的开发和/或使用过程中导致许多相关问题。我们建议开发的软件 (RP-COLUMN) 将 (a) 通过一种或多种分离条件（例如温度、pH 等）的可预测变化，将柱保留差异降至最低，(b) 校正使用过程中柱的变化，(c) 促进实验室之间的方法转移，(d) 允许更可靠地复制文献中的 HPLC 程序，(e) 诊断和纠正由于实验条件选择错误而导致的问题（故障排除），(f) 改进先前开发的程序的分辨率和稳健性，(g) 允许用户表征任何反相色谱柱的选择性，(h) 为不同类型和来源的色谱柱的选择性特性提供数据库，以及 (i) 选择分离条件，以便在与给定程序（HPLC 方法）一起使用时，完全不同的色谱柱将充分发挥作用。这些提出的 RP-COLUMN 九种应用中的每一种都代表了 HPLC 程序开发或使用中重大问题的解决方案。应用（a-f）基于以下事实：保留作为多个变量（条件）同时变化的函数，可以根据少量实验进行预测。应用 (g) 和 (h) 需要深入了解反相 HPLC 的保留过程，而应用 (i) 以完全不同的方式解决色谱柱变异性问题。拟议的商业应用：拟议的产品 RP-COLUMN 将被视为几乎每个进行 HPLC 分析的实验室的重要补充。 HPLC 设备制造商可能希望将该软件的功能合并到他们自己的数据系统和系统控制器中。我们预计初始销售额为 2-5 MM/年。

Manufacturers of alkyl-silica columns (e.g., C8, C18) for high- performance liquid chromatography (HPLC) are currently unable to guarantee that different production batches of nominally equivalent column packing will provide the same retention and separation of different samples. This in turn can lead to a number of related problems during the development and/or use of an HPLC method. Software (RP-COLUMN) that we propose to develop will (a) allow differences in column retention to be minimized by a predictable change in one or more separation conditions (e.g., temperature, pH, etc.), (b) correct for changes in the column during use, (c) facilitate method transfer between laboratories, (d) allow the more reliable duplication of HPLC procedures from the literature, (e) diagnose and correct problems due to error in the selection of experimental conditions (troubleshooting), (f) improve the resolution and robustness of a previously developed procedure, (g) allow the user to characterize any reversed-phase column in terms of its selectivity, (h) provide a data base for the selectivity characteristics of columns of different type and source and (i) select separation conditions such that quite different columns will perform adequately when used with a given procedure (HPLC method). These proposed nine applications of RP- COLUMN each represent a solution to a significant problem in the development or use of HPLC procedures. Applications (a-f) are based on the fact that retention as a function of simultaneous change in several variables (conditions) can be predicted on the basis of a small number of experiments. Applications (g) and (h) require an expanded insight into the retention process for reversed-phase HPLC, while application (i) addresses the problem of column variability in an entirely different fashion. PROPOSED COMMERCIAL APPLICATIONS: The proposed product RP-COLUMN will be regarded as an important addition to virtually every laboratory carrying out HPLC analysis. Manufacturers of HPLC equipment will probably want to incorporate the features of this software into their own data systems and system controllers. We expect initial sale-of $2-5 MM/year.