SOFTWARE FOR IMPROVED USE OF THE COLUMN IN HPLC

烷基-二氧化硅柱的制造商（例如，C8、C18）目前无法保证标称等效色谱柱填料的不同生产批次将对不同样品提供相同的保留和分离。这反过来又会导致在开发和/或使用HPLC方法期间出现许多相关问题。我们建议开发的软件（RP-COLUMN）将（a）通过一个或多个分离条件（例如，温度、pH等），(b)校正使用过程中色谱柱的变化，（c）促进实验室之间的方法转移，（d）允许更可靠地复制文献中的HPLC程序，（e）诊断和校正由于实验条件选择错误而导致的问题（故障排除），（f）提高先前开发的程序的分辨率和鲁棒性，（g）允许用户根据其选择性来表征任何反相柱，（h）为不同类型和来源的色谱柱的选择性特征提供数据库，以及（i）选择分离条件，使得当与给定程序一起使用时，完全不同的色谱柱将充分发挥作用（HPLC方法）。反相柱的这九个应用都代表了HPLC程序开发或使用中一个重要问题的解决方案。应用（a-f）基于这样一个事实，即保留作为几个变量（条件）的同时变化的函数，可以基于少量实验来预测。应用（g）和（h）需要对反相HPLC保留过程的深入了解，而应用（i）以完全不同的方式解决色谱柱可变性问题。拟议的商业应用：拟定产品RP-COLUMN将被视为几乎每个进行HPLC分析的实验室的重要补充。HPLC设备制造商可能希望将该软件的功能整合到他们自己的数据系统和系统控制器中。我们预计初始销售额为200万至500万美元/年。

Manufacturers of alkyl-silica columns (e.g., C8, C18) for high- performance liquid chromatography (HPLC) are currently unable to guarantee that different production batches of nominally equivalent column packing will provide the same retention and separation of different samples. This in turn can lead to a number of related problems during the development and/or use of an HPLC method. Software (RP-COLUMN) that we propose to develop will (a) allow differences in column retention to be minimized by a predictable change in one or more separation conditions (e.g., temperature, pH, etc.), (b) correct for changes in the column during use, (c) facilitate method transfer between laboratories, (d) allow the more reliable duplication of HPLC procedures from the literature, (e) diagnose and correct problems due to error in the selection of experimental conditions (troubleshooting), (f) improve the resolution and robustness of a previously developed procedure, (g) allow the user to characterize any reversed-phase column in terms of its selectivity, (h) provide a data base for the selectivity characteristics of columns of different type and source and (i) select separation conditions such that quite different columns will perform adequately when used with a given procedure (HPLC method). These proposed nine applications of RP- COLUMN each represent a solution to a significant problem in the development or use of HPLC procedures. Applications (a-f) are based on the fact that retention as a function of simultaneous change in several variables (conditions) can be predicted on the basis of a small number of experiments. Applications (g) and (h) require an expanded insight into the retention process for reversed-phase HPLC, while application (i) addresses the problem of column variability in an entirely different fashion. PROPOSED COMMERCIAL APPLICATIONS: The proposed product RP-COLUMN will be regarded as an important addition to virtually every laboratory carrying out HPLC analysis. Manufacturers of HPLC equipment will probably want to incorporate the features of this software into their own data systems and system controllers. We expect initial sale-of $2-5 MM/year.