SOFTWARE FOR IMPROVED USE OF THE COLUMN IN HPLC

用于改进 HPLC 色谱柱使用的软件

• 批准号: 6386508
• 负责人: LLOYD R SNYDER
• 金额: $ 14.98万
• 依托单位: LC RESOURCES, INC.
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2002-11-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6386508
• 关键词: analytical method; computer program /software; computer simulation; computer system design /evaluation; evaluation /testing; high performance liquid chromatography; mathematical model; method development; physical property

Manufacturers of alkyl-silica columns (e.g., C8, C18) for high- performance liquid chromatography (HPLC) are currently unable to guarantee that different production batches of nominally equivalent column packing will provide the same retention and separation of different samples. This in turn can lead to a number of related problems during the development and/or use of an HPLC method. Software (RP-COLUMN) that we propose to develop will (a) allow differences in column retention to be minimized by a predictable change in one or more separation conditions (e.g., temperature, pH, etc.), (b) correct for changes in the column during use, (c) facilitate method transfer between laboratories, (d) allow the more reliable duplication of HPLC procedures from the literature, (e) diagnose and correct problems due to error in the selection of experimental conditions (troubleshooting), (f) improve the resolution and robustness of a previously developed procedure, (g) allow the user to characterize any reversed-phase column in terms of its selectivity, (h) provide a data base for the selectivity characteristics of columns of different type and source and (i) select separation conditions such that quite different columns will perform adequately when used with a given procedure (HPLC method). These proposed nine applications of RP- COLUMN each represent a solution to a significant problem in the development or use of HPLC procedures. Applications (a-f) are based on the fact that retention as a function of simultaneous change in several variables (conditions) can be predicted on the basis of a small number of experiments. Applications (g) and (h) require an expanded insight into the retention process for reversed-phase HPLC, while application (i) addresses the problem of column variability in an entirely different fashion. PROPOSED COMMERCIAL APPLICATIONS: The proposed product RP-COLUMN will be regarded as an important addition to virtually every laboratory carrying out HPLC analysis. Manufacturers of HPLC equipment will probably want to incorporate the features of this software into their own data systems and system controllers. We expect initial sale-of $2-5 MM/year.

用于高效液相色谱的烷基硅柱(例如C8、C18)的制造商目前无法保证不同生产批次的名义上相同的柱填料将对不同的样品提供相同的保留和分离。这进而可能导致在开发和/或使用高效液相方法期间出现许多相关问题。我们建议开发的软件(RP柱)将(A)允许通过一个或多个分离条件(例如，温度、pH等)的可预测变化来最小化柱保留的差异，(B)校正柱在使用期间的变化，(C)促进实验室之间的方法转移，(D)允许更可靠地复制文献中的高效液相程序，(E)诊断和纠正由于实验条件选择中的错误而引起的问题(故障排除)，(F)提高先前开发的程序的分辨率和稳健性，(G)允许用户根据其选择性对任何反相柱进行表征，(H)为不同类型和来源的柱的选择性特性提供数据库，以及(I)选择分离条件，以便完全不同的柱在与特定程序(高效液相色谱方法)一起使用时将充分发挥作用。这九种反相色谱柱的应用，每一种都代表了对开发或使用高效液相程序中的一个重要问题的解决方案。应用(a-f)是基于这样一个事实，即可以在少量实验的基础上预测作为几个变量(条件)同时变化的函数的保留率。应用程序(G)和(H)要求对反相高效液相色谱的保留过程有更深入的了解，而应用程序(I)则以完全不同的方式解决柱的可变性问题。建议的商业应用：建议的产品反相色谱柱将被视为几乎每个进行高效液相分析的实验室的重要补充。高效液相设备的制造商可能希望将该软件的功能整合到他们自己的数据系统和系统控制器中。我们预计最初的销售额为2-500万美元/年。