FIBER OPTIC SENSOR FOR ENVIRONMENTAL PSEUDOESTROGENS

用于环境假雌激素的光纤传感器

• 批准号: 6204646
• 负责人: RICHARD H SMITH
• 金额: $ 1.18万
• 依托单位: IA, INC.
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-06-20 至 2000-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6204646
• 关键词: bioassay; biomedical equipment development; biosensor device; environmental toxicology; estrogen analog; estrogen receptors; fiber optics; receptor binding

DESCRIPTION (Adapted from applicant's abstract): In response to increasing awareness of the suspected role of estrogen mimics in reproductive disorders and cancer, during Phase I work, the investigators demonstrated the feasibility of developing a fiber optic sensor for screening estrogen mimics. The instrument reports rapid real-time data on binding between biological macromolecules and their ligands. Sensor measurements performed on several estrogens and mimics were well correlated with conventional affinity measurements for those compounds. By combining information obtained from two different types of fibers, the sensors distinguished between strong and weak estrogens and between estrogenic agonists and an estrogenic antagonist, reflecting strong agreement between sensor results and bioactivity. A patent and three publications are in preparation as a result of this work. Proposed Phase II work will entail: 1) optimization of chemical and molecular biological methods and protocols, 2) refinements in mathematical methods, 3) prototype design and construction, and 4) validation with blind samples of estrogen mimics. The resulting instrument and stabilized reagents provide a rapid assessment of estrogenic and endocrine disrupting potential of samples. This approach may be adapted to pharmaceutical development and identification of non- estrogenic compounds with endocrine-disrupting properties. PROPOSED COMMERCIAL APPLICATION: Agencies monitoring environmental estrogen mimics and screening compounds for estrogenic endocrine disrupting potential, industries monitoring their own waste streams for pseudo-estrogens, pharmaceutical companies seeking anti-breast cancer agents by screening compounds for binding to the estrogen receptor or determining real time binding constants of substances for biomolecules, researchers studying mechanisms of signal transduction.

描述（改编自申请人摘要）：回应