DRUGS OF ABUSE IN SALIVA

唾液中的滥用药物

• 批准号: 3507115
• 负责人: RICHARD H SMITH
• 金额: $ 24.1万
• 依托单位: PACIFIC BIOMETRICS, INC.
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-08-01 至 1994-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3507115
• 关键词: cocaine; drug detection; mass spectrometry; saliva; ultrafiltration

This research project is designed to investigate the role of saliva as an alternative biological fluid for the quantitative measurement of cocaine and its metabolites. Specimen collection is based on an osmotic device for the collection of an ultrafiltrate of saliva in the mouth. The studies will provide a model for investigations of other drugs of abuse in an ultrafiltrate of saliva. Preliminary results obtained in Phase I of the study demonstrated the feasibility of screening for cocaine and its metabolite, benzoylecgonine, in saliva if the drug was ingested within 24 h of sample collection. During Phase II, sample collection will be expanded to determine how long cocaine use can be detected using an ultrafiltrate of saliva. At the same time, analytical methods will be developed that focus on the lower concentration of analytes and the relatively small sample volume available with saliva as sample medium. These methods will include immunochemical screening procedures and confirmatory analysis by GC/MS or LC/MS. We will investigate whether the ratio of different cocaine metabolites in saliva can be used to obtain additional information about the time of drug ingestion. The refined analytical methods will be used to make an attempt to suggest threshold levels in saliva to determine recent use. The established criteria will be tested in a mixed population of drug users and non-users by comparing urinary concentrations of cocaine and benzoylecgonine with those in saliva.

这项研究项目旨在调查唾液作为一种 用于可卡因定量测量的替代生物流体 及其代谢物。 样本采集基于渗透装置， 口腔中唾液超滤液的收集。 研究 将为调查其他滥用药物提供一个模式， 唾液的超滤液。 第一阶段的初步结果 研究表明，筛查可卡因及其 如果在24小时内摄入药物，唾液中的代谢产物苯甲酰芽子碱 样本收集。 在第二阶段，将扩大样本采集 以确定使用超滤液可以检测可卡因使用多长时间 口水 与此同时，将制定分析方法， 分析物浓度较低，样品相对较小， 以唾液作为样本介质。 这些方法将包括 免疫化学筛选程序和GC/MS确证性分析，或 我们将研究不同可卡因的比例是否 唾液中的代谢物可用于获得关于 药物摄入的时间 将使用改进的分析方法， 尝试提出唾液中的阈值水平，以确定最近的 使用. 将在混合药物人群中对既定标准进行检测 使用者和非使用者通过比较可卡因和 苯甲酰芽子碱与唾液中的那些。