DRUGS OF ABUSE IN SALIVA

这项研究项目旨在调查唾液作为一种 替代生物液用于可卡因的定量检测 及其代谢物。样本采集是基于渗透装置 唾液在口腔中的超滤性聚集。这些研究 将为其他滥用药物的调查提供一个模式 唾液超滤液。第一阶段取得的初步成果 研究论证了可卡因及其相关物质筛查的可行性 如果药物在24小时内摄入，唾液中的代谢物苯甲酰ecGonine 样本采集。在第二阶段，样本收集将扩大 为了确定使用可卡因的超滤液可以检测到可卡因的使用时间 唾液。与此同时，将发展分析方法，重点是 关于较低的分析物浓度和相对较小的样本 容量以唾液为样品介质。这些方法将包括 免疫化学筛选程序和GC/MS或确证分析 LC/MS我们将调查不同可卡因的比例 唾液中的代谢物可以用来获得关于 吸食药物的时间。精细化的分析方法将用于 尝试建议唾液中的阈值水平以确定最近 使用。已建立的标准将在混合药物人群中进行测试 通过比较可卡因和非可卡因的尿液浓度 与唾液中的苯甲酰ecGonine相同。

This research project is designed to investigate the role of saliva as an alternative biological fluid for the quantitative measurement of cocaine and its metabolites. Specimen collection is based on an osmotic device for the collection of an ultrafiltrate of saliva in the mouth. The studies will provide a model for investigations of other drugs of abuse in an ultrafiltrate of saliva. Preliminary results obtained in Phase I of the study demonstrated the feasibility of screening for cocaine and its metabolite, benzoylecgonine, in saliva if the drug was ingested within 24 h of sample collection. During Phase II, sample collection will be expanded to determine how long cocaine use can be detected using an ultrafiltrate of saliva. At the same time, analytical methods will be developed that focus on the lower concentration of analytes and the relatively small sample volume available with saliva as sample medium. These methods will include immunochemical screening procedures and confirmatory analysis by GC/MS or LC/MS. We will investigate whether the ratio of different cocaine metabolites in saliva can be used to obtain additional information about the time of drug ingestion. The refined analytical methods will be used to make an attempt to suggest threshold levels in saliva to determine recent use. The established criteria will be tested in a mixed population of drug users and non-users by comparing urinary concentrations of cocaine and benzoylecgonine with those in saliva.