PROTEIN TARGETING TO THE TOXOPLASMA GONDII PLASTID

针对弓形虫质体的蛋白质

• 批准号: 2887673
• 负责人: Marilyn Parsons
• 金额: $ 8.95万
• 依托单位: SEATTLE BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2887673
• 关键词: Toxoplasma gondii; bacterial proteins; complementary DNA; epitope mapping; human tissue; lyase; molecular cloning; nucleic acid sequence; organelles; polymerase chain reaction; protein biosynthesis; protein structure function; protein transport; transcription factor; transfection; translation factor; western blottings

Among the opportunistic infections in AIDS patients are those caused by two related parasites: Toxoplasma gondii and Cryptosporidium parvum. Both organisms belong to the phylum Apicomplexa, whose members (including the causative agent of malaria) have recently been shown to contain a unique plastid-like organelle. Because humans do not have a homologous organelle, the plastid may provide a unique target for the development of drugs that act specifically against these parasites. However, little is known about the protein constituents of this plasmid or its function. T. gondii is the only apicomplexan parasite for which genetic approaches are well-developed. This proposal describes a plan to examine the localization of proteins to the apicomplexan plastid using T. gondii as our model. We will obtain the sequence of the entire coding region of a cDNA predicted to specify a nuclearly encoded plastid protein. We will then verify that the protein is indeed a plastid resident, using an epitope tagging/transfection approach. Finally we will determine if sequences from the plastid protein can route a reporter molecule to the plastid. These studies will provide the first information about the biogenesis of the apicomplexan plastid and lay the groundwork for studying plastid function.

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