MOLECULAR AND QUANTITATIVE VARIATION IN AE ALBOPICTUS

本提案的目标是评估快速范围扩张的遗传后果，并评估一种携带疾病的蚊子的微生境专门化。亚洲虎蚊白纹伊蚊是登革热的重要媒介，也能传播黄热病和各种北美原生虫媒病毒。白纹伊蚊于1980年代中期在德克萨斯州休斯顿附近被引入美国，其分布现在从佛罗里达州延伸到伊利诺伊州和俄亥俄州。比较祖先（TX）和衍生（FL， OH）种群内和种群间的变异，将阐明在北美该物种的范围扩张过程中，连锁位点的遗传漂变、迁移和选择是如何分布变异的。为了评估分子变异，将开发AFLP标记，并将其用于表征整个北美地区以及轮胎育种和树洞育种群体之间的群体结构。对该物种在北美的快速入侵和气候适应的核心数量性状的遗传变异和分化进行了研究，并用于研究AFLP标记的变异和分化在多大程度上准确预测了数量性状的变异和分化。

The goals of this proposal are to evaluate the genetic consequences of a rapid range expansion and to assess micro- habitat specialization within a species of disease carrying mosquito. The Asian tiger mosquito, Aedes albopictus, is an important vector of dengue fever and is also able to transmit yellow fever and a variety of native North American arboviruses. Aedes albopictus was introduced into the United States in the vicinity of Houston, TX, in the mid- 1980's, and its distribution now extends from Florida to Illinois and Ohio. Comparing within- and between-population variability among ancestral (TX) and derived (FL, OH) populations will elucidate how genetic drift, migration, and selection at linked loci have distributed variability during the range expansion of this species in North America. In order to assess molecular variation, AFLP markers will be developed and used to characterize population structure throughout North America, and between tire breeding and tree-hole breeding populations. Genetic variation and differentiation of quantitative characters central to the rapid invasion and climatic adaptation of this species in North America will be examined, and used to investigate the extent to which variation and differentiation of AFLP markers accurately predicts variation and differentiation of quantitative characters.