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Massively parallel sequencing of the diapause transcriptome in Aedes albopictus

白纹伊蚊滞育转录组的大规模并行测序

基本信息

批准号：
7871476
负责人：
PETER ARMBRUSTER
金额：
$ 15.06万
依托单位：
GEORGETOWN UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-06-19 至 2012-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7871476
关键词：
Acyl Coenzyme A Aedes American Arboviruses Asians Bees Bioinformatics Biological Biological Models Cattle Chemicals Chikungunya virus Communities Cues Culicidae DNA Data Databases Dengue Development Diapause Disease Outbreaks Elements Embryo Environment Event Evolution Female Foundations Funding Mechanisms Future Gene Expression Gene Expression Profile Genes Genetic Genome Genomics Goals Graafian Follicles Honey Hydrocarbons Insecta Invaded Knowledge Larva Lead Measures Mediating Molecular Molecular Probes Nature Oocytes Pattern Photoperiod Physiological Physiological Processes Physiology Population Production Public Health RNA Interference Relative (related person)Research Resources Reverse Transcriptase Polymerase Chain Reaction Role Specialist Surface Technology Testing Tigers Tissues Transcript Water Yellow Fever base biological adaptation to stress day length egg experience gene discovery genome database human disease long chain fatty acid novel novel strategies programs public health relevance research study response trait vector vector control

项目摘要

DESCRIPTION (provided by applicant): The Asian tiger mosquito, Aedes albopictus (Skuse), is an important vector of dengue fever and also efficiently transmits yellow fever, chikungunya virus and several native North American arboviruses. The rapid spread of this mosquito from its native Asian range across the globe during the last 20 years and its recent involvement in disease outbreaks represents an outstanding public health concern. Photoperiodically mediated egg diapause is a pivotal ecological trait central to the ability of A. albopictus and other medically relevant mosquitoes to inhabit temperate environments and spread across broad geographic ranges. The long-term goal of this research program is to rigorously define the molecular basis of photoperiodic diapause in A. albopictus as a foundation for developing novel forms of vector control based on the disruption of this critical ecological adaptation. The immediate goal of this proposal is to identify transcriptional elements of the photoperiodic diapause response in A. albopictus. In Specific Aim One, massively parallel pyrosequencing and bioinformatics analyses will be used to compare diapause vs. non-diapause transcriptomes from oocytes, developing embryos, and pharate larvae. In Specific Aim 2, quantitative RT- PCR will be employed to identify transcriptional events underpinning the diapause response by comparing gene expression between a diapausing (temperate) and non- diapausing (tropical) population. In addition to providing a foundation for developing novel forms of vector control, results from these studies will rapidly advance gene discovery and understanding of stress response physiology in mosquitoes. PUBLIC HEALTH RELEVANCE Photoperiodic diapause refers to the ability of insects to measure day length (photoperiod) as a cue for initiating seasonally appropriate patterns of developmental arrest. This study will investigate the molecular control of photoperiodic diapause in the invasive and medically important mosquito, Aedes albopictus. Results of this study will provide a foundation for developing novel strategies for vector control based on the disruption of this critical ecological adaptation.

描述（由申请人提供）：亚洲虎蚊白纹伊蚊（Skuse）是登革热的重要媒介，也有效传播黄热病、基孔肯雅病毒和几种北美本土虫媒病毒。在过去的20年里，这种蚊子从其原产地亚洲范围迅速传播到全球地球仪，最近参与疾病爆发，这是一个突出的公共卫生问题。光周期介导的卵滞育是一个重要的生态学特性，它决定了A.白纹伊蚊和其他医学相关的蚊子栖息在温带环境中，并在广泛的地理范围内传播。本研究计划的长期目标是严格定义光周期滞育的分子基础。白纹伊蚊作为一个基础，开发新形式的病媒控制的基础上破坏这一关键的生态适应。本研究的近期目标是确定光周期滞育反应的转录元件。白纹伊蚊在具体目标一，大规模并行焦磷酸测序和生物信息学分析将被用来比较滞育与非滞育转录组从卵母细胞，发育中的胚胎，和pharate幼虫。在具体目标2中，将采用定量RT-PCR通过比较滞育（温带）和非滞育（热带）种群之间的基因表达来鉴定支持滞育反应的转录事件。除了为开发新形式的病媒控制提供基础外，这些研究的结果将迅速推进基因发现和对蚊子应激反应生理学的理解。光周期滞育是指昆虫测量日长（光周期）的能力，作为启动季节性发育停滞模式的线索。本研究旨在探讨白纹伊蚊光周期滞育的分子调控机制。这项研究的结果将提供一个基础，为制定新的战略，病媒控制的基础上破坏这一关键的生态适应。

项目成果

期刊论文数量（7）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

An experimental and bioinformatics protocol for RNA-Seq analyses of photoperiodic diapause in the Asian tiger mosquito, Aedes albopictus.

DOI：
10.3791/51961
发表时间：
2014-11
期刊：
Journal of visualized experiments : JoVE
影响因子：
0
作者：
Monica F. Poelchau;Xin Huang;Allison C. Goff;Julie A. Reynolds;P. Armbruster
通讯作者：
Monica F. Poelchau;Xin Huang;Allison C. Goff;Julie A. Reynolds;P. Armbruster

Transcript profiling reveals mechanisms for lipid conservation during diapause in the mosquito, Aedes albopictus.

DOI：
10.1016/j.jinsphys.2012.04.013
发表时间：
2012-07
期刊：
JOURNAL OF INSECT PHYSIOLOGY
影响因子：
2.2
作者：
Reynolds, Julie A.;Poelchau, Monica F.;Rahman, Zahra;Armbruster, Peter A.;Denlinger, David L.
通讯作者：
Denlinger, David L.

A de novo transcriptome of the Asian tiger mosquito, Aedes albopictus, to identify candidate transcripts for diapause preparation.