GENE EXPRESSION IN ANTIGEN SPECIFIC LYMPHOCYTES DURING V

CD8+T细胞在控制病毒以及细胞内细菌和寄生虫感染方面发挥着关键作用。近年来，大量证据表明CD8+T细胞在控制肿瘤生长中起着关键作用。细胞免疫的CD8成分的多种功能是由三种不同的CD8+T细胞执行的：幼稚细胞、效应细胞和记忆细胞。然而，人们对病毒感染期间抗原特异性淋巴细胞中基因表达的变化知之甚少。急性感染伴随着幼稚细胞的大量扩张而成为效应细胞。抗原清除后，95-98%的效应细胞将死亡。存活的细胞是记忆细胞，将为小鼠的生命提供保护性免疫。我们将通过对使用重组MHC I类四聚体分离的抗原特异性淋巴细胞进行定量逆转录-聚合酶链式反应(qRT-PCR)来确定基因表达的变化。这种试剂将允许我们从生理相关的情况下分离抗原特异性细胞，而不需要体外操作。对基因表达的分析将使我们能够制定合理的策略来剖析CD8功能的分子机制，这对疫苗接种和免疫治疗至关重要。

CD8+ T cells play a critical role in controlling viral as well as intracellular bacterial and parasitic infections. In recent years considerable evidence has accumulated demonstrating the key role CD8+ T cells play in controlling tumor growth. The multiple functions of the CD8 component of cellular immunity are executed by three different classes of CD8 + T cells: naive, effector, and memory cells. Little, however is known about the changes' of gene expression that occur in antigen specific lymphocytes during viral infection. Acute infection is accompanied by massive expansion of naive cells to become effector cells. After antigen clearance 95- 98% of the effector cells will die. The surviving cells are memory cells and will confer protective immunity for the life of the mouse. We will determine changes in gene expression by performing quantitative Reverse Transcription-Polymerase Chain Reaction (qRT- PCR) on antigen specific lymphocytes isolated using recombinant MHC class I tetramers. This reagent will allow us to isolate antigen specific cells from a physiologically relevant situation without in vitro manipulation. Analysis of gene expression will allow us to develop rational strategies to dissect molecular mechanisms of CD8 function which is critical for vaccinations and immunotherapies.