GENE EXPRESSION IN ANTIGEN SPECIFIC LYMPHOCYTES DURING V
V 期间抗原特异性淋巴细胞的基因表达
基本信息
- 批准号:6372894
- 负责人:
- 金额:$ 1.87万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2001
- 资助国家:美国
- 起止时间:2001-08-01 至
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
CD8+ T cells play a critical role in controlling viral as well as intracellular bacterial and parasitic infections. In recent years considerable evidence has accumulated demonstrating the key role CD8+ T cells play in controlling tumor growth. The multiple functions of the CD8 component of cellular immunity are executed by three different classes of CD8 + T cells: naive, effector, and memory cells. Little, however is known about the changes' of gene expression that occur in antigen specific lymphocytes during viral infection. Acute infection is accompanied by massive expansion of naive cells to become effector cells. After antigen clearance 95- 98% of the effector cells will die. The surviving cells are memory cells and will confer protective immunity for the life of the mouse. We will determine changes in gene expression by performing quantitative Reverse Transcription-Polymerase Chain Reaction (qRT- PCR) on antigen specific lymphocytes isolated using recombinant MHC class I tetramers. This reagent will allow us to isolate antigen specific cells from a physiologically relevant situation without in vitro manipulation. Analysis of gene expression will allow us to develop rational strategies to dissect molecular mechanisms of CD8 function which is critical for vaccinations and immunotherapies.
CD8+ T细胞在控制病毒以及细胞内细菌和寄生虫感染方面发挥着关键作用。近年来积累的大量证据表明CD8+ T细胞在控制肿瘤生长中起关键作用。细胞免疫中CD8成分的多种功能由三种不同类型的CD8 + T细胞执行:初始细胞、效应细胞和记忆细胞。然而,对于病毒感染期间抗原特异性淋巴细胞中基因表达的变化知之甚少。急性感染伴随着幼稚细胞大量扩增成为效应细胞。抗原清除后,95% - 98%的效应细胞会死亡。存活下来的细胞是记忆细胞,将为小鼠的一生提供保护性免疫。我们将通过对重组MHC I类四聚体分离的抗原特异性淋巴细胞进行定量逆转录-聚合酶链反应(qRT- PCR)来确定基因表达的变化。该试剂将使我们能够从生理相关情况下分离抗原特异性细胞,而无需体外操作。基因表达的分析将使我们能够制定合理的策略来解剖CD8功能的分子机制,这对疫苗接种和免疫治疗至关重要。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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JASON Mitchell GRAYSON其他文献
JASON Mitchell GRAYSON的其他文献
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{{ truncateString('JASON Mitchell GRAYSON', 18)}}的其他基金
Repurposed Drugs to Inhibit Human T Cells and Autoimmunity
重新利用药物抑制人类 T 细胞和自身免疫
- 批准号:
8889627 - 财政年份:2014
- 资助金额:
$ 1.87万 - 项目类别:
Repurposed Drugs to Inhibit Human T Cells and Autoimmunity
重新利用药物抑制人类 T 细胞和自身免疫
- 批准号:
8771735 - 财政年份:2014
- 资助金额:
$ 1.87万 - 项目类别:
Interferon Alpha Regulation of Murine Gammaherpesvirus Latency
干扰素α对鼠丙型疱疹病毒潜伏期的调节
- 批准号:
8648978 - 财政年份:2011
- 资助金额:
$ 1.87万 - 项目类别:
Mechanisms of CD8+ T Cell Apoptosis During Acute and Chronic Viral Infections
急慢性病毒感染过程中CD8 T细胞凋亡机制
- 批准号:
8018876 - 财政年份:2010
- 资助金额:
$ 1.87万 - 项目类别:
Redox control of CD8 T cell proliferation, differentiation and death
氧化还原控制 CD8 T 细胞增殖、分化和死亡
- 批准号:
8240546 - 财政年份:2008
- 资助金额:
$ 1.87万 - 项目类别:
Redox control of CD8 T cell proliferation, differentiation and death
氧化还原控制 CD8 T 细胞增殖、分化和死亡
- 批准号:
7556319 - 财政年份:2008
- 资助金额:
$ 1.87万 - 项目类别:
Redox control of CD8 T cell proliferation, differentiation and death
氧化还原控制 CD8 T 细胞增殖、分化和死亡
- 批准号:
7783793 - 财政年份:2008
- 资助金额:
$ 1.87万 - 项目类别:
Redox control of CD8 T cell proliferation, differentiation and death
氧化还原控制 CD8 T 细胞增殖、分化和死亡
- 批准号:
7464853 - 财政年份:2008
- 资助金额:
$ 1.87万 - 项目类别:
Redox control of CD8 T cell proliferation, differentiation and death
氧化还原控制 CD8 T 细胞增殖、分化和死亡
- 批准号:
8034740 - 财政年份:2008
- 资助金额:
$ 1.87万 - 项目类别:
GENE EXPRESSION IN ANTIGEN SPECIFIC LYMPHOCYTES DURING V
V 期间抗原特异性淋巴细胞的基因表达
- 批准号:
6169102 - 财政年份:2000
- 资助金额:
$ 1.87万 - 项目类别:
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