IMPROVE HAPTEN DESIGN FOR ANTICOCAINE CATALYTIC ANTIBODY

改进抗古卡因催化抗体的半抗原设计

• 批准号: 6175053
• 负责人: Milan N Stojanovic
• 金额: $ 4.09万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-03-15 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6175053
• 关键词: abzyme; analog; antibody formation; biotechnology; cocaine; drug addiction antagonist; drug design /synthesis /production; enzyme activity; haptens; hydrolysis; immunization; laboratory mouse; synthetic antigens

DESCRIPTION (Applicant's Abstract): Catalytic antibodies that degrade cocaine have potential as anti-addiction therapeutic agents. Nine such catalysts have been previously elicited and cloned and are being mutagenized abused to improve catalytic activity. However, the synthesis of transition-states analog is the first, critical step in the creation of catalytic antibodies and two new strategies have recently been reported for the generation of antibodies with especially high catalytic activity - reactive immunization and heterologous immunization. Utilizing these strategies, the design and synthesized novel transition-state analogs for the hydrolysis of the benzoyl ester of cocaine is proposed. These analogs are expected to elicit antibodies with potent catalytic mechanisms, such as acid-base catalysis or covalent catalysis, and high activity for the hydrolysis of cocaine.

描述（申请人摘要）：