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MORPHOLOGICAL CORRELATES OF LONG TERM SENSITIZATION

长期致敏的形态相关性

基本信息

批准号：
6185150
负责人：
MARCY L WAINWRIGHT
金额：
$ 2.01万
依托单位：
UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
依托单位国家：
美国
项目类别：
财政年份：
2000
资助国家：
美国
起止时间：
2000-09-01 至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/6185150
关键词：
Aplysia afferent nerve behavioral /social science research tag behavioral habituation /sensitization cell component structure /function confocal scanning microscopy long term memory motor neurons neural plasticity reflex

项目摘要

DESCRIPTION (Adapted from applicant's abstract): One of the fundamental problems in neurobiology is to understand the cellular and molecular events underlying learning and memory. Considerable progress has been made in understanding the neural basis of simple forms of learning in the marine mollusk, Aplysia, through the study of two simple defensive withdrawal reflexes, tail siphon withdrawal and gill siphon withdrawal. These reflexes can be modified by sensitization, a simple form of non-associative learning in which the behavioral response to a mild stimulus is enhanced following the presentation of another, usually noxious, stimulus. Previous studies suggest that one general correlate of sensitization is the modification of neuronal structure. However, this assumption may be oversimplified. Preliminary studies suggest that there may be multiple forms of long-term sensitization, and that morphological changes are only induced by the more present forms of this memory. The specific aims of this research project are: 1) to determine the time course of long-term sensitization in the tail induced siphon withdrawal reflex; 2) to determine the conditions that induce a form of long-term sensitization associated with morphological changes; and 3) to examine the functional role of neuronal outgrowth in long-term sensitization. The effects of various training protocols on both the behavioral response of the reflex, and on the morphology of the sensory neurons mediating this response will be analyzed. Changes in neuronal morphology will be analyzed using 3-D reconstructions of the neuronal structure. In addition, we will use confocal microscopy to examine whether neuronal outgrowth is contributing to the behavioral response by strengthening pre-existing connections to motor neurons, or by adding new connections to the neuronal circuitry underlying the response. Through the quantitative analysis of neuronal morphology we hope to provide a more complete framework for a structural model of learning and memory.

描述(改编自申请人的摘要)：基本原则之一神经生物学的问题是理解细胞和分子事件潜在的学习和记忆。在……方面取得了相当大的进展理解海洋中简单形式学习的神经基础通过研究软体动物、海兔两种简单的防御性撤退反射、尾部虹吸式退缩和鳃虹吸式退缩。这些反应可以通过敏感化进行修改，这是一种简单的非联想学习形式其中对轻微刺激的行为反应在以下情况下得到增强另一种通常有害的刺激的表现。以前的研究认为敏化的一个普遍相关性是对神经元结构。然而，这种假设可能过于简单化了。初步研究表明，可能存在多种形式的长期敏化，形态变化只由更多的呈现这种记忆的形式。这项研究项目的具体目的主要有：1)确定尾部长期敏化的时间进程诱导虹吸戒断反射；2)确定诱发的条件一种与形态变化相关的长期敏化形式； 3)研究神经元突起在长期中的功能作用敏化。不同训练方案对两种运动能力的影响反射的行为反应以及对感觉形态的影响我们将对参与这种反应的神经元进行分析。神经细胞的变化形态分析将使用神经元的3D重建结构。此外，我们将使用共聚焦显微镜来检查神经元的生长通过以下方式对行为反应做出贡献加强已有的运动神经元连接，或通过添加新的与反应背后的神经回路的连接。通过我们希望对神经元形态的定量分析提供更多的学习和记忆结构模型的完整框架。